HRP-Linked Polyclonal Antibody to Apolipoprotein B100 (APOB100) | MyBioSource MBS2044850 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HRP-Linked Polyclonal Antibody to Apolipoprotein B100 (APOB100)

catalog :

MBS2044850

quantity :

0.1 mg

price :

210 USD

clonality :

polyclonal

host :

rabbit

conjugate :

HRP

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2044850

products type :

Antibody

products full name :

HRP-Linked Polyclonal Antibody to Apolipoprotein B100 (APOB100)

products short name :

[Apolipoprotein B100 (APOB100)]

products name syn :

[Apo-B100]

other names :

[apolipoprotein B-100; Apolipoprotein B-100; apolipoprotein B-100; apolipoprotein B]

products gene name :

[APOB100]

other gene names :

[APOB; APOB; FLDB; LDLCQ4; apoB-48; apoB-100; Apo B-100; Apo B-48]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific Affinity Chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

0.74mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC.

app notes :

Western blotting: 0.5-2 Ug/mL;1:370-1400. Immunohistochemistry: 5-20 Ug/mL;1:37-140. Immunocytochemistry: 5-20 Ug/mL;1:37-140. Optimal working dilutions must be determined by end user.

other info1 :

Organism Species: Homo sapiens (Human). Source: Antibody labeling. Label: HRP. Traits: Liquid. Immunogen: Recombinant APOB100 (His3365~Glu3548) expressed in E.coli.

other info2 :

Unconjugated Antibody: The unconjugated antibody version of this item is also available as catalog #MBS2004161

ncbi gi num :

105990532

ncbi acc num :

NP_000375.2

ncbi gb acc num :

NM_000384.2

uniprot acc num :

P04114

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA1 Transcription Factor Network Pathway (137979); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (106028); LDL-mediated Lipid Transport Pathway (106159)

ncbi summary :

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA- UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

uniprot summary :

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. Protein type: Carrier; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2p24.1. Cellular Component: actin cytoskeleton; cell soma; chylomicron; cytoplasm; cytosol; early endosome; endoplasmic reticulum lumen; endoplasmic reticulum membrane; endosome membrane; extracellular region; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; plasma membrane. Molecular Function: cholesterol transporter activity; heparin binding; low-density lipoprotein receptor binding; phospholipid binding; protein binding. Biological Process: cellular protein catabolic process; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; leukocyte migration; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein metabolic process; receptor-mediated endocytosis; response to virus; retinoid metabolic process. Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1

size1 :

0.1 mg

price1 :

210 USD

size2 :

0.2 mg

price2 :

310

size3 :

1 mg

price3 :

735

more info or order :

MyBioSource product webpage