LAT, 28-233aa, Human,His tag, E Coli | MyBioSource MBS204482 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

LAT, 28-233aa, Human,His tag, E Coli

catalog :

MBS204482

quantity :

0.05 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204482

products type :

Recombinant Protein

products full name :

LAT, 28-233aa, Human,His tag, E Coli

products short name :

LAT

products name syn :

linker for activation of T-cells family member 1 isoform b; LAT1; pp36

other names :

linker for activation of T-cells family member 1 isoform b; Linker for activation of T-cells family member 1; linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells, transmembrane adaptor; p36-38; linker for activation of T cells; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38

products gene name :

LAT

other gene names :

LAT; LAT; LAT1; pp36; pp36

uniprot entry name :

LAT_HUMAN

sequence length :

233

sequence :

MGSSHHHHHH SSGLVPRGSH MHCHRLPGSY DSTSSDSLYP RGIQFKRPHT VAPWPPAYPP VTSYPPLSQP DLLPIPRSPQ PLGGSHRTPS SRRDSDGANS VASYENEEPA CEDADEDEDD YHNPGYLVVL PDSTPATSTA APSAPALSTP GIRDSAFSME SIDDYVNVPE SGESAEASLD GSREYVNVSQ ELHPGAAKTE PAALSSQEAE EVEEEGAPDY ENLQELN

purity :

> 95 % by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 1mM DTT

concentration :

1 mg/ml (determined by Bradford)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Signal Transduction

products description :

LAT is an integral membrane protein which is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells. Recombinant human LAT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Paz PE., et al. (2001) Biochem J. 356(2):461-71. Zhang W., et al. (1998) Cell. 92(1):83-92.

ncbi gi num :

62739155

ncbi acc num :

NP_001014987

ncbi gb acc num :

NM_001014987.1

ncbi mol weight :

24.4 kDa (227aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

ncbi pathways :

Adaptive Immune System Pathway (366160); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); FCERI Mediated Ca+2 Mobilization Pathway (833827); FCERI Mediated MAPK Activation Pathway (833826); Fc Epsilon RI Signaling Pathway (83082); Fc Epsilon RI Signaling Pathway (493); Fc Epsilon Receptor (FCERI) Signaling Pathway (833825); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590)

ncbi summary :

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

LAT: an adaptor protein involved in T-cell antigen receptor (TCR) signal transduction pathway. Plays an important role downstream of protein tyrosine kinases. Essential for TCR-mediated Ras-Erk activation and calcium flux. Protein type: Adaptor/scaffold; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: signalosome; mast cell granule; plasma membrane; integral to membrane; immunological synapse; intercellular junction; lipid raft. Molecular Function: protein binding; SH3/SH2 adaptor activity; protein kinase binding. Biological Process: integrin-mediated signaling pathway; platelet activation; T cell activation; positive regulation of signal transduction; calcium-mediated signaling; mast cell degranulation; T cell receptor signaling pathway; Ras protein signal transduction; innate immune response; gene expression; immune response; inflammatory response; blood coagulation; lymphocyte homeostasis; regulation of T cell activation

size1 :

0.05 mg

price1 :

310 USD

size2 :

0.25 mg

price2 :

720

more info or order :

MyBioSource product webpage