BCAT1, 1-386aa, Human, His tag, E Coli | MyBioSource MBS204479 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

BCAT1, 1-386aa, Human, His tag, E Coli

catalog :

MBS204479

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204479

products type :

Recombinant Protein

products full name :

BCAT1, 1-386aa, Human, His tag, E Coli

products short name :

BCAT1

products name syn :

Branched chain amino-acid transaminase; cytosolic; BCATC; BCT1; ECA39; MECA39; PNAS121; PP18

other names :

branched-chain-amino-acid aminotransferase, cytosolic isoform 1; Branched-chain-amino-acid aminotransferase, cytosolic; branched-chain-amino-acid aminotransferase, cytosolic; branched chain aminotransferase 1, cytosolic; placental protein 18; branched chain amino-acid transaminase 1, cytosolic; Protein ECA39

products gene name :

BCAT1

other gene names :

BCAT1; BCAT1; BCT1; PP18; BCATC; ECA39; MECA39; PNAS121; BCT1; ECA39; BCAT(c)

uniprot entry name :

BCAT1_HUMAN

sequence length :

386

sequence :

MGSSHHHHHH SSGLVPRGSH MGSMKDCSNG CSAECTGEGG SKEVVGTFKA KDLIVTPATI LKEKPDPNNL VFGTVFTDHM LTVEWSSEFG WEKPHIKPLQ NLSLHPGSSA LHYAVELFEG LKAFRGVDNK IRLFQPNLNM DRMYRSAVRA TLPVFDKEEL LECIQQLVKL DQEWVPYSTS ASLYIRPTFI GTEPSLGVKK PTKALLFVLL SPVGPYFSSG TFNPVSLWAN PKYVRAWKGG TGDCKMGGNY GSSLFAQCEA VDNGCQQVLW LYGEDHQITE VGTMNLFLYW INEDGEEELA TPPLDGIILP GVTRRCILDL AHQWGEFKVS ERYLTMDDLT TALEGNRVRE MFGSGTACVV CPVSDILYKG ETIHIPTMEN GPKLASRILS KLTDIQYGRE ESDWTIVLS

purity :

> 90% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

BCAT1, also known as branched chain amino-acid transaminase, cytosolic, catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Recombinant human BCAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Goto M., et al. (2005) J. Biol. Chem. 280:37246-37256. Schuldiner O., et al. (1996) Proc. Natl. Acad. Sci. u.S.A. 93:7143-7148

ncbi gi num :

38176287

ncbi acc num :

NP_005495

ncbi gb acc num :

NM_005504.6

ncbi mol weight :

45.4 kDa (409aa), confirmed by MALDI-TOF

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Branched-chain Amino Acid Catabolism Pathway (106179); Leucine Degradation, Leucine = Acetoacetate + Acetyl-CoA Pathway (413354); Leucine Degradation, Leucine = Acetoacetate + Acetyl-CoA Pathway (468229); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)

ncbi summary :

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]

uniprot summary :

BCAT1: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Transferase; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 2.6.1.42. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: mitochondrion; cytosol. Molecular Function: identical protein binding; branched-chain-amino-acid transaminase activity. Biological Process: cell proliferation; branched chain family amino acid catabolic process; G1/S transition of mitotic cell cycle; branched chain family amino acid biosynthetic process

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage