protein

TK1, 1-234aa, Human, His tag, E Coli

MBS204448

0.1 mg

310 USD

Recombinant Protein

TK1, 1-234aa, Human, His tag, E Coli

TK1

Thymidine kinase; cytosolic; TK2

thymidine kinase, cytosolic; Thymidine kinase, cytosolic; thymidine kinase, cytosolic; thymidine kinase 1 soluble isoform; thymidine kinase-1; thymidine kinase 1, soluble

TK1

TK1; TK1; TK2

KITH_HUMAN

234

MGSSHHHHHH SSGLVPRGSH MGSHMSCINL PTVLPGSPSK TRGQIQVILG PMFSGKSTEL MRRVRRFQIA QYKCLVIKYA KDTRYSSSFC THDRNTMEAL PACLLRDVAQ EALGVAVIGI DEGQFFPDIV EFCEAMANAG KTVIVAALDG TFQRKPFGAI LNLVPLAESV VKLTAVCMEC FREAAYTKRL GTEKEVEVIG GADKYHSVCR LCYFKKASGQ PAGPDNKENC PVPGKPGEAV AARKLFAPQQ ILQCSPAN

> 90% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Epigenomics

TK1, also known as thymidine kinase, is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase. It is present in two forms in mammalian cells, TK1 and TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Deoxythymidine is present in the body fluids as a result of degradation of DNA from food and from dead cells. Thymidine kinase is required for the action of many antiviral drugs. It is used to select hybridoma cell lines in production of monoclonal antibodies. In clinical chemistry it is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease, mainly of hematological malignancies. Recombinant human TK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Chang Z.F., et al. (1998) J. Biol. Chem. 273:12095-12100. Kreidberg J.A., et al. (1986) Mol. Cell. Biol. 6:2903-2909

164698438

NP_003249

NM_003258.4

28.0 kDa (258aa) confirmed by MALDI-TOF

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309)

TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells. Protein type: Nucleotide Metabolism - pyrimidine; EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Kinase, other. Chromosomal Location of Human Ortholog: 17q23.2-q25.3. Cellular Component: cytosol. Molecular Function: identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding. Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; deoxyribonucleoside monophosphate biosynthetic process; protein homotetramerization; fetal process involved in parturition; response to cortisol stimulus; gut development; response to copper ion; pyrimidine base metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; thymidine metabolic process; pyrimidine nucleoside salvage; phosphorylation

0.1 mg

310 USD

0.5 mg

720