KARS, 63-597aa, Human, His tag, E Coli | MyBioSource MBS204431 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

KARS, 63-597aa, Human, His tag, E Coli

catalog :

MBS204431

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204431

products type :

Recombinant Protein

products full name :

KARS, 63-597aa, Human, His tag, E Coli

products short name :

KARS

products name syn :

Lysyl-tRNA synthetase; CMTRIB; KARS2; KIAA0070; KRS

other names :

lysine--tRNA ligase isoform 2; Lysine--tRNA ligase; lysine--tRNA ligase; lysRS; lysine tRNA ligase; lysyl-tRNA synthetase; Lysyl-tRNA synthetase; LysRS

products gene name :

KARS

other gene names :

KARS; KARS; KRS; KARS1; KARS2; CMTRIB; DFNB89; KIAA0070; LysRS

uniprot entry name :

SYK_HUMAN

sequence length :

597

sequence :

MGSSHHHHHH SSGLVPRGSH MGSGVGPEEE SVDPNQYYKI RSQAIHQLKV NGEDPYPHKF HVDISLTDFI QKYSHLQPGD HLTDITLKVA GRIHAKRASG GKLIFYDLRG EGVKLQVMAN SRNYKSEEEF IHINNKLRRG DIIGVQGNPG KTKKGELSII PYEITLLSPC LHMLPHLHFG LKDKETRYRQ RYLDLILNDF VRQKFIIRSK IITYIRSFLD ELGFLEIETP MMNIIPGGAV AKPFITYHNE LDMNLYMRIA PELYHKMLVV GGIDRVYEIG RQFRNEGIDL THNPEFTTCE FYMAYADYHD LMEITEKMVS GMVKHITGSY KVTYHPDGPE GQAYDVDFTP PFRRINMVEE LEKALGMKLP ETNLFETEET RKILDDICVA KAVECPPPRT TARLLDKLVG EFLEVTCINP TFICDHPQIM SPLAKWHRSK EGLTERFELF VMKKEICNAY TELNDPMRQR QLFEEQAKAK AAGDDEAMFI DENFCTALEY GLPPTAGWGM GIDRVAMFLT DSNNIKEVLL FPAMKPEDKK ENVATTDTLE STTVGTSV

purity :

> 95% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

concentration :

1.0 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Enzymes & Proteases

products description :

KARS, also known as Lysyl-tRNA synthetase, belongs to the class-II aminoacyl-tRNA synthetase family. It exists as both mitochondrial and cytoplasmic isoforms produced by alternative splicing, and thought to play a role in autoimmune diseases, such as polymyositis or dermatomyositis. This protein functions to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Recombinant human KARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Targoff I.N. et al. (1993) J. Clin. Invest. 91: 2556-2564. Tolkunova E. et al. (2000) J. Biol. Chem. 275: 35063-35069.

ncbi gi num :

5031815

ncbi acc num :

NP_005539

ncbi gb acc num :

NM_005548.2

ncbi mol weight :

63.7 kDa (558aa)

ncbi pathways :

Aminoacyl-tRNA Biosynthesis Pathway (83030); Aminoacyl-tRNA Biosynthesis Pathway (424); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (413439); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (468360); Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); Mitochondrial TRNA Aminoacylation Pathway (105982); TRNA Aminoacylation Pathway (105980)

ncbi summary :

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.1.1.6; Ligase; Mitochondrial; Translation. Chromosomal Location of Human Ortholog: 16q23.1. Cellular Component: microtubule cytoskeleton; aminoacyl-tRNA synthetase multienzyme complex; mitochondrion; mitochondrial matrix; cytoplasm; plasma membrane; extracellular region; nucleus; cytosol. Molecular Function: amino acid binding; protein binding; metal ion binding; lysine-tRNA ligase activity; ATP binding; tRNA binding. Biological Process: tRNA aminoacylation for protein translation; viral reproduction; lysyl-tRNA aminoacylation; tRNA processing; gene expression; diadenosine tetraphosphate biosynthetic process. Disease: Deafness, Autosomal Recessive 89; Charcot-marie-tooth Disease, Recessive Intermediate B

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage