product summary
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company name :
MyBioSource
product type :
protein
product name :
ADA, 1-363aa, Human, His tag, E Coli
catalog :
MBS204402
quantity :
0.05 mg
price :
375 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS204402
products type :
Recombinant Protein
products full name :
ADA, 1-363aa, Human, His tag, E Coli
products short name :
ADA
products name syn :
Adenosine deaminase; Adenosine aminohydrolase; ADA1
other names :
adenosine deaminase; Adenosine deaminase; adenosine deaminase; adenosine aminohydrolase; adenosine deaminase; Adenosine aminohydrolase
products gene name :
ADA
other gene names :
ADA; ADA; ADA1
uniprot entry name :
ADA_HUMAN
sequence length :
363
sequence :
MGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL
purity :
> 85% by SDS - PAGE
form :
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli. Bioactivity: Specific activity: approximately >25 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C. Assay: 1. Prerapre a 1.5 ml reaction mix: the final concentrations are 53.3mM potassium phosphate, 0.045mM adenosine, 0.003% (w/v) bovine serum. 2. Add recombinant ADA protein with various concentrations (0.1ug, 0.2) in assay buffer. 3. Mix by inversion and record A260nm for approximately 5 minutes.
products categories :
Enzymes & Proteases
products description :
ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Recombinant human ADA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
products references :
Gines S., et al. (2002) Biochem. 361:203-209. Daddona P.E., et al. (1984) J. Biol. Chem. 259:12101-12106
ncbi gi num :
47078295
ncbi acc num :
NP_000013
ncbi gb acc num :
NM_000022.2
ncbi mol weight :
42.9 kDa (383aa), confirmed by MALDI-TOF
ncbi pathways :
C-MYB Transcription Factor Network Pathway 138073!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Nucleotides Pathway 106263!!Primary Immunodeficiency Pathway 83125!!Primary Immunodeficiency Pathway 537!!Purine Metabolism Pathway 82944!!Purine Metabolism Pathway 106265!!Purine Metabolism Pathway 307!!Purine Salvage Pathway 106273
ncbi summary :
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]
size :
0.05 mg
price :
375 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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