protein

BCAT2, 28-392aa, Human, His tag, E Coli

MBS204383

0.05 mg

375 USD

Recombinant Protein

BCAT2, 28-392aa, Human, His tag, E Coli

BCAT2

Branched chain amino-acid aminotransferase 2; mitochondrial; BCAM; BCATM; BCT2; PP18

branched-chain-amino-acid aminotransferase, mitochondrial isoform a; Branched-chain-amino-acid aminotransferase, mitochondrial; branched-chain-amino-acid aminotransferase, mitochondrial; branched chain aminotransferase 2, mitochondrial; placental protein 18; branched chain amino-acid transaminase 2, mitochondrial; Placental protein 18; PP18

BCAT2

BCAT2; BCAT2; BCAM; BCT2; PP18; BCATM; BCATM; BCT2; ECA40; BCAT(m); PP18

BCAT2_HUMAN

392

MGSSHHHHHH SSGLVPRGSH MGSHMASSSF KAADLQLEMT QKPHKKPGPG EPLVFGKTFT DHMLMVEWND KGWGQPRIQP FQNLTLHPAS SSLHYSLQLF EGMKAFKGKD QQVRLFRPWL NMDRMLRSAM RLCLPSFDKL ELLECIRRLI EVDKDWVPDA AGTSLYVRPV LIGNEPSLGV SQPTRALLFV ILCPVGAYFP GGSVTPVSLL ADPAFIRAWV GGVGNYKLGG NYGPTVLVQQ EALKRGCEQV LWLYGPDHQL TEVGTMNIFV YWTHEDGVLE LVTPPLNGVI LPGVVRQSLL DMAQTWGEFR VVERTITMKQ LLRALEEGRV REVFGSGTAC QVCPVHRILY KDRNLHIPTM ENGPELILRF QKELKEIQYG IRAHEWMFPV

> 90% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH 7.5) containing 10% glycerol 0.2M NaCl, 1mM DTT

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Metabolism

BCAT2 belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. This protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. It may also function as a transporter of branched chain alpha-keto acids. Recombinant human BCAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Coles SJ. et al. (2009) Biochemistry Jan 27;48(3):645-56. Yennawar N.H.et al. (2007) J. Biol. Chem. 281:39660-39671

50658084

NP_001181

NM_001190.3

43.9 kDa(390aa), confirmed by MALDI-TOF

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Branched-chain Amino Acid Catabolism Pathway (106179); Leucine Degradation, Leucine = Acetoacetate + Acetyl-CoA Pathway (413354); Leucine Degradation, Leucine = Acetoacetate + Acetyl-CoA Pathway (468229); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

BCAT2: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Homodimer. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.6.1.42; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Transferase; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine degradation. Chromosomal Location of Human Ortholog: 19q13. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: branched-chain-amino-acid transaminase activity. Biological Process: valine metabolic process; branched chain family amino acid catabolic process; leucine metabolic process; branched chain family amino acid biosynthetic process; isoleucine catabolic process

0.05 mg

375 USD

0.25 mg

925