TPSAB1, 31-275aa, Human, His tag, E Coli | MyBioSource MBS204320 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

TPSAB1, 31-275aa, Human, His tag, E Coli

catalog :

MBS204320

quantity :

0.05 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204320

products type :

Recombinant Protein

products full name :

TPSAB1, 31-275aa, Human, His tag, E Coli

products short name :

TPSAB1

products name syn :

Tryptase alpha/beta-1; TPS1; TPS2; TPSB1

other names :

tryptase alpha/beta-1; Tryptase alpha/beta-1; tryptase alpha/beta-1; mast cell alpha II tryptase; mast cell beta I tryptase; tryptase I; tryptase alpha II; tryptase alpha-1; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase-1; tryptase-I; tryptase-III; tryptase alpha/beta 1; Tryptase I; Tryptase alpha-1

products gene name :

TPSAB1

other gene names :

TPSAB1; TPSAB1; TPS1; TPS2; TPSB1; TPS1; TPS2; TPSB1; Tryptase-1

uniprot entry name :

TRYB1_HUMAN

sequence length :

275

sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMIVGGQ EAPRSKWPWQ VSLRVHGPYW MHFCGGSLIH PQWVLTAAHC VGPDVKDLAA LRVQLREQHL YYQDQLLPVS RIIVHPQFYT AQIGADIALL ELEEPVNVSS HVHTVTLPPA SETFPPGMPC WVTGWGDVDN DERLPPPFPL KQVKVPIMEN HICDAKYHLG AYTGDDVRIV RDDMLCAGNT RRDSCQGDSG GPLVCKVNGT WLQAGVVSWG EGCAQPNRPG IYTRVTYYLD WIHHYVPKKP

purity :

> 85% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M urea

concentration :

1.0 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

TPSAB1, also known as tryptase alpha/beta-1, is a tryptase that is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known an endogenous proteinase inhibitor. This protein has been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human TPSAB1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

products references :

Tanaka T. et al. (1983) J Biol Chem. 258(22):13552-7. Marquardt u. et al. (2002) J Mol Biol. 321(3):491-502.

ncbi gi num :

13775595

ncbi acc num :

NP_003285

ncbi gb acc num :

NM_003294.3

ncbi mol weight :

30.1 kDa(270aa)

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

uniprot summary :

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.59. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular matrix; extracellular space; extracellular region. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response; proteolysis

size1 :

0.05 mg

price1 :

310 USD

size2 :

0.25 mg

price2 :

720

more info or order :

MyBioSource product webpage