PAPSS1, 24-624aa, Human, His tag, E Coli | MyBioSource MBS204296 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

PAPSS1, 24-624aa, Human, His tag, E Coli

catalog :

MBS204296

quantity :

0.1 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204296

products type :

Recombinant Protein

products full name :

PAPSS1, 24-624aa, Human, His tag, E Coli

products short name :

PAPSS1

products name syn :

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; ATPSK1; PAPSS; SK1

other names :

bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1; PAPS synthase 1; PAPSS 1; SK 1; sulfurylase kinase 1; 3'-phosphoadenosine 5'-phosphosulfate synthase 1; Sulfurylase kinase 1; SK 1; SK1

products gene name :

PAPSS1

other gene names :

PAPSS1; PAPSS1; SK1; PAPSS; ATPSK1; ATPSK1; PAPSS; PAPS synthase 1; PAPSS 1; SK 1; SK1; SAT

uniprot entry name :

PAPS1_HUMAN

sequence length :

624

sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMRATNV TYQAHHVSRN KRGQVVGTRG GFRGCTVWLT GLSGAGKTTV SMALEEYLVC HGIPCYTLDG DNIRQGLNKN LGFSPEDREE NVRRIAEVAK LFADAGLVCI TSFISPYTQD RNNARQIHEG ASLPFFEVFV DAPLHVCEQR DVKGLYKKAR AGEIKGFTGI DSEYEKPEAP ELVLKTDSCD VNDCVQQVVE LLQERDIVPV DASYEVKELY VPENKLHLAK TDAETLPALK INKVDMQWVQ VLAEGWATPL NGFMREREYL QCLHFDCLLD GGVINLSVPI VLTATHEDKE RLDGCTAFAL MYEGRRVAIL RNPEFFEHRK EERCARQWGT TCKNHPYIKM VMEQGDWLIG GDLQVLDRVY WNDGLDQYRL TPTELKQKFK DMNADAVFAF QLRNPVHNGH ALLMQDTHKQ LLERGYRRPV LLLHPLGGWT KDDDVPLMWR MKQHAAVLEE GVLNPETTVV AIFPSPMMYA GPTEVQWHCR ARMVAGANFY IVGRDPAGMP HPETGKDLYE PSHGAKVLTM APGLITLEIV PFRVAAYNKK KKRMDYYDSE HHEDFEFISG TRMRKLAREG QKPPEGFMAP KAWTVLTEYY KSLEKA

purity :

> 85% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Enzymes & Proteases

products description :

PAPSS1, also known as bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway, yielding 3'-phosphoadenylylsulfate (PAPS). PAPSS1 is also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. Recombinant human PAPSS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Girard J.-P., et al. (1998) FASEB J. 12:603-612. Venkatachalam K.V., et al. (1999) J. Biol. Chem. 274:2601-2604

ncbi gi num :

46094058

ncbi acc num :

NP_005434

ncbi gb acc num :

NM_005443.4

ncbi mol weight :

70.9 kDa (626aa)

ncbi pathways :

Biological Oxidations Pathway (105698); Cytosolic Sulfonation Of Small Molecules Pathway (105724); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective B3GAT3 Causes JDSSDHD Pathway (1127588); Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway (1127592); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1127587); Defective CHST14 Causes EDS, Musculocontractural Type Pathway (1127591); Defective CHST3 Causes SEDCJD Pathway (1127590); Defective CHST6 Causes MCDC1 Pathway (1127593); Defective CHSY1 Causes TPBS Pathway (1127589)

ncbi summary :

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008]

uniprot summary :

PAPSS1: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5 -phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3 -phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. Protein type: Energy Metabolism - sulfur; Nucleotide Metabolism - purine; EC 2.7.1.25; EC 2.7.7.4; Other Amino Acids Metabolism - selenoamino acid; Kinase, other. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: cytosol. Molecular Function: adenylylsulfate kinase activity; sulfate adenylyltransferase (ATP) activity; ATP binding; nucleotidyltransferase activity. Biological Process: sulfate assimilation; glycosaminoglycan metabolic process; xenobiotic metabolic process; carbohydrate metabolic process; pathogenesis; phosphorylation; skeletal development; 3 -phosphoadenosine 5 -phosphosulfate metabolic process; 3 -phosphoadenosine 5 -phosphosulfate biosynthetic process

size1 :

0.1 mg

price1 :

375 USD

size2 :

0.5 mg

price2 :

925

more info or order :

MyBioSource product webpage