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company name :
MyBioSource
product type :
protein
product name :
PPME1, 1-386aa, Human, His tag, E Coli
catalog :
MBS204278
quantity :
0.1 mg
price :
310 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS204278
products type :
Recombinant Protein
products full name :
PPME1, 1-386aa, Human, His tag, E Coli
products short name :
PPME1
products name syn :
Protein phosphatase methylesterase 1; FLJ22226; PME-1
other names :
protein phosphatase methylesterase 1 isoform a; Protein phosphatase methylesterase 1; protein phosphatase methylesterase 1; protein phosphatase methylesterase-1; protein phosphatase methylesterase 1
products gene name :
PPME1
other gene names :
PPME1; PPME1; PME-1; PME1; PME-1
uniprot entry name :
PPME1_HUMAN
sequence length :
386
sequence :
MGSSHHHHHH SSGLVPRGSH MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII SRVQCRIVAL DLRSHGETKV KNPEDLSAET MAKDVGNVVE AMYGDLPPPI MLIGHSMGGA IAVHTASSNL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC
purity :
> 95% by SDS - PAGE
form :
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Signal Transduction
products description :
PPME1, also known protein phosphatase methylesterase-1(PME1), catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. It can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. It is conserved from yeast to human and contains a motif found in lipases having a catalytic triad activated serine as their active site nucleophile. Recombinant human PPME1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
products references :
Ogris E. et al. (1999) J. Biol. Chem. 274: 14382-14391. Gagnon S.N. et al. (2002) Biochem. J. 368:263-271.
ncbi gi num :
7706645
ncbi acc num :
NP_057231
ncbi gb acc num :
NM_016147.2
ncbi mol weight :
44.4 kDa(406aa), confirmed by MALDI-TOF
ncbi summary :
This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
uniprot summary :
PPME1: Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme. Belongs to the AB hydrolase superfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.1.89; Motility/polarity/chemotaxis; Hydrolase; Protein phosphatase, regulatory subunit. Chromosomal Location of Human Ortholog: 11q13.4. Molecular Function: protein phosphatase 2A binding; protein C-terminal methylesterase activity; protein phosphatase type 2A regulator activity; protein phosphatase inhibitor activity; protein phosphatase binding. Biological Process: regulation of catalytic activity; negative regulation of catalytic activity; protein amino acid demethylation
size1 :
0.1 mg
price1 :
310 USD
size2 :
0.5 mg
price2 :
720
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
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