protein

DuSP3, 1-185aa, Human, His tag, E Coli

MBS204275

0.1 mg

310 USD

Recombinant Protein

DuSP3, 1-185aa, Human, His tag, E Coli

DuSP3

Dual specificity protein phosphatase 3; VHR

dual specificity protein phosphatase 3; Dual specificity protein phosphatase 3; dual specificity protein phosphatase 3; dual specificity protein phosphatase VHR; serine/threonine specific protein phosphatase; vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; dual specificity phosphatase 3; Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR

DuSP3

DUSP3; DUSP3; VHR; VHR; VHR

DUS3_HUMAN

185

MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP

> 95 % by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.

1 mg/ml (determined by Bradford)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 degree C, pH7.5 using 10mM of substrate. Assay: 1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 10mM Tris (pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP. 2. Pre-warm reaction mix in heat block at 37C. 3. Add recombinant DuSP3 protein solution with various concentrations (1ug, 2ug, 3ug) in 200ul reaction buffer. 4. Mix by inversion and incubate at 37C for 10minutes. 5. Stop the reaction by adding the 800ul of 0.1N NaOH and record the absorbance at 405nm.

Cancer

DuSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DuSP3 is expressed in both breast and ovarian tissues. DuSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Recombinant human DuSP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Todd JL., et al. (1999) J Biol Chem. 274(19):13271-80. Keyse SM., et al. (1995) Biochim Biophys Acta. 1265(2-3):152-60.

4758208

NP_004081

NM_004090.3

22.6 kDa (205aa), confirmed by MALDI-TOF

Activated TLR4 Signalling Pathway (106400); ERK/MAPK Targets Pathway (106482); ERKs Are Inactivated Pathway (106483); Immune System Pathway (106386); Innate Immune System Pathway (106387); MAP Kinase Activation In TLR Cascade Pathway (160135); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); MAPK Targets/ Nuclear Events Mediated By MAP Kinases Pathway (160138); MyD88 Cascade Initiated On Plasma Membrane Pathway (205107)

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]

DUSP3: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP3 inactivates the mitogen-activated kinases Erk2 and Jnk and is phosphorylated by ZAP-70. Phosphorylation by ZAP-70 is required for DUSP3 to inhibit the Erk2-Elk-1 pathway. Protein type: EC 3.1.3.16; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: nucleoplasm; cytoplasm; immunological synapse; cytosol; nucleus. Molecular Function: MAP kinase phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity. Biological Process: negative regulation of JNK cascade; nerve growth factor receptor signaling pathway; negative regulation of MAPKKK cascade; in utero embryonic development; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; negative regulation of T cell receptor signaling pathway; toll-like receptor 3 signaling pathway; positive regulation of mitotic cell cycle; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; negative regulation of T cell activation; inactivation of MAPK activity

0.1 mg

310 USD

0.5 mg

720