protein

JAM3, 32-241aa Human, His tag, E Coli

MBS204249

0.02 mg

310 USD

Recombinant Protein

JAM3, 32-241aa Human, His tag, E Coli

JAM3

Junctional adhesion molecule C; Junctional adhesion molecule 3; JAM-C; JAMC

junctional adhesion molecule C isoform 1; Junctional adhesion molecule C; junctional adhesion molecule C; junctional adhesion molecule 3; JAM-2; Junctional adhesion molecule 3; JAM-3

JAM3

JAM3; JAM3; JAMC; JAM-2; JAM-3; JAM-C; JAM-C; JAM-3

JAM3_HUMAN

310

MGSSHHHHHH SSGLVPRGSH MGSMVNLKSS NRTPVVQEFE SVELSCIITD SQTSDPRIEW KKIQDEQTTY VFFDNKIQGD LAGRAEILGK TSLKIWNVTR RDSALYRCEV VARNDRKEID EIVIELTVQV KPVTPVCRVP KAVPVGKMAT LHCQESEGHP RPHYSWYRND VPLPTDSRAN PRFRNSSFHL NSETGTLVFT AVHKDDSGQY YCIASNDAGS ARCEEQEMEV YDLN

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM EDTA, 5mM DTT

0.25 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. JAM3 is localized in the tight junctions between high endothelial cells. unlike other proteins in this family, this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. JAM3 is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. Recombinant human JAM3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Santoso S., et al. (2002). J Exp Med. 2;196(5):679-91.

328927055

NP_116190

NM_032801.4

26 kDa (234aa), confirmed by MALDI-TOF

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (83102); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (515); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028); Integrin Cell Surface Interactions Pathway (106110); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494)

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

JAM3: Participates in cell-cell adhesion. It is a counter- receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. Defects in JAM3 are the cause of hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC). A syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain tissue, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 11q25. Cellular Component: Golgi apparatus; extracellular space; desmosome; tight junction; integral to membrane; plasma membrane. Molecular Function: integrin binding; protein binding; protein homodimerization activity; protein heterodimerization activity. Biological Process: myelination; adaptive immune response; extracellular matrix organization and biogenesis; axon regeneration; cell-matrix adhesion; transmission of nerve impulse; neutrophil homeostasis; leukocyte migration during inflammatory response; myeloid progenitor cell differentiation; establishment of cell polarity; angiogenesis; blood coagulation; leukocyte migration; spermatid development. Disease: Hemorrhagic Destruction Of The Brain, Subependymal Calcification, And Cataracts

0.02 mg

310 USD

0.1 mg

720