protein

CCND2, 1-289aa, Human, His tag, E Coli

MBS204235

0.1 mg

310 USD

Recombinant Protein

CCND2, 1-289aa, Human, His tag, E Coli

CCND2

Cyclin D2; KIAK0002; MGC102758; G1/S-specific cyclin-D2

G1/S-specific cyclin-D2; G1/S-specific cyclin-D2; G1/S-specific cyclin-D2; cyclin D2

CCND2

CCND2; CCND2; MPPH3; KIAK0002

CCND2_HUMAN

289

MGSSHHHHHH SSGLVPRGSH MGSHMELLCH EVDPVRRAVR DRNLLRDDRV LQNLLTIEER YLPQCSYFKC VQKDIQPYMR RMVATWMLEV CEEQKCEEEV FPLAMNYLDR FLAGVPTPKS HLQLLGAVCM FLASKLKETS PLTAEKLCIY TDNSIKPQEL LEWELVVLGK LKWNLAAVTP HDFIEHILRK LPQQREKLSL IRKHAQTFIA LCATDFKFAM YPPSMIATGS VGAAICGLQQ DEEVSSLTCD ALTELLAKIT NTDVDCLKAC QEQIEAVLLN SLQQYRQDQR DGSKSEDELD QASTPTDVRD IDL

> 85% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 1M urea.

1 mg/ml (determined by Bradford)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Cancer

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of this protein was observed in ovarian and testicular tumors. Recombinant human CCND2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Inaba T., et al. (1992) Genomics. 13(3):565-74. Wang Z., et al. (2008) Cell Struct Funct. 33(2):171-83.

4502617

NP_001750

NM_001759.3

35.6 kDa (313aa)

B Cell Receptor Signaling Pathway (198909); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cell Cycle - G1/S Transition Pathway (1084768); Cell Cycle - G1/S Transition Pathway (1108231); Cyclin D Associated Events In G1 Pathway (105767); DNA Damage Response Pathway (198788)

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3). [provided by RefSeq, Sep 2014]

CCND2: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/p27Kip1, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/p27Kip1 required for nuclear translocation and modulation of CDK4-mediated kinase activity. Belongs to the cyclin family. Cyclin D subfamily. Protein type: Cell cycle regulation; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: nucleoplasm; nuclear membrane; cyclin-dependent protein kinase holoenzyme complex; nucleolus; chromatin; cytosol; nucleus. Molecular Function: protein binding; protein kinase binding. Biological Process: positive regulation of cyclin-dependent protein kinase activity; cell division; positive regulation of protein amino acid phosphorylation; cell cycle. Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 3

0.1 mg

310 USD

0.5 mg

720