catalog number :
MBS204230
products type :
Recombinant Protein
products full name :
EMCN, 19-190aa, Human, His tag, E Coli,
products short name :
EMCN
products name syn :
Endomucin; EMCN2; MuC14
other names :
endomucin isoform 1; Endomucin; endomucin; MUC-14; endomucin-2; gastric cancer antigen Ga34; mucin-14; endomucin; Endomucin-2; Gastric cancer antigen Ga34; Mucin-14; MUC-14
products gene name :
EMCN
other gene names :
EMCN; EMCN; EMCN2; MUC14; EMCN2; MUC14; MUC-14
uniprot entry name :
MUCEN_HUMAN
sequence :
MGSSHHHHHH SSGLVPRGSH MGSHMNSTGV LEAANNSLVV TTTKPSITTP NTESLQKNVV TPTTGTTPKG TITNELLKMS LMSTATFLTS KDEGLKATTT DVRKNDSIIS NVTVTSVTLP NAVSTLQSSK PKTETQSSIK TTEIPGSVLQ PDASPSKTGT LTSIPVTIPE NTSQSQVIGT EGGKNASTSA TSRSYSS
purity :
> 90% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 100mM NaCl
concentration :
0.25mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Cytoskeleton
products description :
Endomucin, also called endothelial sialomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. This protein expressed in heart, kidney and lung. Recombinant human EMCN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Kinoshita M, et al. (2001) FEBS Lett 499 (1-2): 121-6. Kuhn A., et al. (2002) J. Invest. Dermatol. 119:1388-1393
ncbi gb acc num :
NM_016242.3
ncbi mol weight :
20.5kDa (197aa), confirmed by MALDI-TOF
ncbi summary :
EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM, Mar 2008]
uniprot summary :
EMCN: Endothelial sialomucin, also called endomucin or mucin- like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: integral to membrane; plasma membrane; extracellular region