protein

DPPA3, 1-159aa, Human, His tag, E Coli

MBS204224

0.05 mg

375 USD

Recombinant Protein

DPPA3, 1-159aa, Human, His tag, E Coli

DPPA3

Developmental pluripotency associated 3; STELLA

developmental pluripotency-associated protein 3; Developmental pluripotency-associated protein 3; developmental pluripotency-associated protein 3; stella-related protein; developmental pluripotency associated 3; Stella-related protein

DPPA3

DPPA3; DPPA3; STELLA; STELLAR

DPPA3_HUMAN

159

MGSSHHHHHH SSGLVPRGSH MGSMDPSQFN PTYIPGSPQM LTEENSRDDS GASQISSETL IKNLSNLTIN ASSESVSPLS EALLRRESVG AAVLREIEDE WLYSRRGVRT LLSVQREKMA RLRYMLLGGV RTHERRPTNK EPKGVKKESR PFKCPCSFCV SNGWDPSENA RIGNQDTKPL QP

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol,

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Development

DPPA3 is a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this protein may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. Recombinant human DPPA3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Nakamura,T.,et al. (2007) Nat. Cell Biol. 9 (1), 64-71

40548326

NP_954980

NM_199286.2

20.2 kDa (182aa)

This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008]

DPPA3: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at Lys-9 (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: male pronucleus; cytoplasm; female pronucleus; nucleus. Molecular Function: methylated histone residue binding. Biological Process: chromatin modification; embryonic cleavage

0.05 mg

375 USD

0.25 mg

925