protein

TDG, 1-410aa, Human, His tag, E Coli

MBS204223

0.1 mg

310 USD

Recombinant Protein

TDG, 1-410aa, Human, His tag, E Coli

TDG

Thymine-DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase

Thymine-DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase; thymine-DNA glycosylase; Thymine-DNA glycosylase; hTDG

TDG

TDG; TDG; hTDG; hTDG

TDG_HUMAN

410

MGSSHHHHHH SSGLVPRGSH MGSMEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4 urea, 10% glycerol, 0.1M NaCl

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Epigenomics

Thymine-DNA glycosylase, also known as TDG, belongs to the TDG/mug DNA glycosylase family. TDG is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. Recombinant human TDG protein, fused to His-tag at N-terminus, was expressed in E.coli

um S., et al. (1998) J Biol Chem. 273:20728-20736. Hardeland u., et al. (2002) EMBO J. 21:1456-1464.

22902196

AAH37557

48.4 kDa (433aa)

Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Pyrimidine Pathway (105845); DNA Repair Pathway (105837); Depyrimidination Pathway (105843); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Epigenetic Regulation Of Gene Expression Pathway (1127671)

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]

TDG: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T C/T T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. Belongs to the TDG/mug DNA glycosylase family. Protein type: Nuclear receptor co-regulator; DNA repair, damage; Hydrolase; EC 3.2.2.29. Chromosomal Location of Human Ortholog: 12q24.1. Cellular Component: nucleoplasm; PML body; plasma membrane; nucleus. Molecular Function: protein domain specific binding; protein binding; structure-specific DNA binding; protein homodimerization activity; SUMO binding; protein kinase C binding; DNA N-glycosylase activity; double-stranded DNA binding; damaged DNA binding; transcription factor binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; mismatched DNA binding. Biological Process: transcription from RNA polymerase II promoter; embryonic development; mismatch repair; base-excision repair, AP site formation; base-excision repair; depyrimidination; gene expression; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; DNA repair; negative regulation of protein binding; regulation of gene expression, epigenetic

0.1 mg

310 USD

0.5 mg

720