ALDOB, 1-364aa, Human, His tag, E Coli | MyBioSource MBS204182 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

ALDOB, 1-364aa, Human, His tag, E Coli

catalog :

MBS204182

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204182

products type :

Recombinant Protein

products full name :

ALDOB, 1-364aa, Human, His tag, E Coli

products short name :

ALDOB

products name syn :

Fructose-bisphosphate aldolase B; ALDB; ALDO2; Fructose bisphosphate aldolase B

other names :

fructose-bisphosphate aldolase B; Fructose-bisphosphate aldolase B; fructose-bisphosphate aldolase B; aldolase 2; aldolase B, fructose-bisphosphatase; liver-type aldolase; aldolase B, fructose-bisphosphate; Liver-type aldolase

products gene name :

ALDOB

other gene names :

ALDOB; ALDOB; ALDB; ALDO2; ALDB

uniprot entry name :

ALDOB_HUMAN

sequence length :

364

sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMAHRFP ALTQEQKKEL SEIAQSIVAN GKGILAADES VGTMGNRLQR IKVENTEENR RQFREILFSV DSSINQSIGG VILFHETLYQ KDSQGKLFRN ILKEKGIVVG IKLDQGGAPL AGTNKETTIQ GLDGLSERCA QYKKDGVDFG KWRAVLRIAD QCPSSLAIQE NANALARYAS ICQQNGLVPI VEPEVIPDGD HDLEHCQYVT EKVLAAVYKA LNDHHVYLEG TLLKPNMVTA GHACTKKYTP EQVAMATVTA LHRTVPAAVP GICFLSGGMS EEDATLNLNA INLCPLPKPW KLSFSYGRAL QASALAAWGG KAANKEATQE AFMKRAMANC QAAKGQYVHT GSSGAASTQS LFTACYTY

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

Fructose-bisphosphate aldolase B, also known as ALDOB, is one of three known aldolase isoenzymes, and is found in kidney and small adult intestine where it is associated with aldolases A or C. ALDOB catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is regulated by the hormones Insulin and glucagon and has been implicated in hereditary fructose intolerance disease. Recombinant human ALDOB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Caffe A R., et al. (1994) J Comp Neurol. 348:291-297. Dehnes Y., et al. (1998) J Neurosci. 18:3606-3619.

ncbi gi num :

40354205

ncbi acc num :

NP_000026

ncbi gb acc num :

NM_000035.3

ncbi mol weight :

42 kDa (388aa) confirmed by MALDI-TOF

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Fructose Catabolism Pathway (106218); Gluconeogenesis Pathway (106204)

ncbi summary :

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]

uniprot summary :

ALDOB: Defects in ALDOB are the cause of hereditary fructose intolerance (HFI). HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Belongs to the class I fructose-bisphosphate aldolase family. Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - fructose and mannose; EC 4.1.2.13; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase. Chromosomal Location of Human Ortholog: 9q21.3-q22.2. Cellular Component: microtubule organizing center; cytosol. Molecular Function: identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; ATPase binding. Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; NADH oxidation; positive regulation of ATPase activity; carbohydrate metabolic process; glucose metabolic process; pathogenesis; fructose catabolic process; fructose metabolic process; gluconeogenesis. Disease: Fructose Intolerance, Hereditary

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage