protein

ADH1A, 1-375aa, Human, His tag, E Coli

MBS204173

0.1 mg

310 USD

Recombinant Protein

ADH1A, 1-375aa, Human, His tag, E Coli

ADH1A

Alcohol dehydrogenase 1A; ADH1

alcohol dehydrogenase 1A; Alcohol dehydrogenase 1A; alcohol dehydrogenase 1A; ADH, alpha subunit; alcohol dehydrogenase 1 (class I), alpha polypeptide; alcohol dehydrogenase subunit alpha; aldehyde reductase; alcohol dehydrogenase 1A (class I), alpha polypeptide; Alcohol dehydrogenase subunit alpha

ADH1A

ADH1A; ADH1A; ADH1; ADH1

ADH1A_HUMAN

375

MGSSHHHHHH SSGLVPRGSH MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAVGICGTD DHVVSGTMVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLAIPQCGK CRICKNPESN YCLKNDVSNP QGTLQDGTSR FTCRRKPIHH FLGISTFSQY TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG LGGVGLSAIM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSMNPMLLL TGRTWKGAIL GGFKSKECVP KLVADFMAKK FSLDALITHV LPFEKINEGF DLLHSGKSIR TILMF

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Metabolism

Alcohol dehydrogenase 1A, also known as ADH1A, belongs to the alcohol dehydrogenase family. ADH1 is a monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. ADH1A plays a major role in ethanol metabolism. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH1A in the liver is the removal of ethanol formed by microorganisms in the intestinal tract. Recombinant human ADH1A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Jelski W., et al. (2007) Dig Dis Sci. 52:1513-1516. Smith M., et al. (1973) Ann Hum Genet. 36:401-414.

4501929

NP_000658

NM_000667.3

42 kDa (395aa) confirmed by MALDI-TOF

Abacavir Metabolism Pathway 645351!!Abacavir Transport And Metabolism Pathway 645349!!Biological Oxidations Pathway 105698!!Chemical Carcinogenesis Pathway 673221!!Chemical Carcinogenesis Pathway 673237!!Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway 1127640!!Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway 1127641!!Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway 1127642!!Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway 1127643!!Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway 1127644

This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

0.1 mg

310 USD