MBD3, 1-291aa, Human, His tag, E Coli | MyBioSource MBS204158 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MBD3, 1-291aa, Human, His tag, E Coli

catalog :

MBS204158

quantity :

0.05 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204158

products type :

Recombinant Protein

products full name :

MBD3, 1-291aa, Human, His tag, E Coli

products short name :

MBD3

products name syn :

Methyl-CpG-binding domain protein 3; Methyl CpG binding domain protein 3; Methyl-CpG-binding protein MBD3

other names :

Methyl-CpG-binding domain protein 3; methyl-CpG-binding domain protein 3; methyl-CpG binding domain protein 3; Methyl-CpG-binding protein MBD3

products gene name :

MBD3

other gene names :

MBD3; MBD3

uniprot entry name :

MBD3_HUMAN

sequence length :

259

sequence :

MGSSHHHHHH SSGLVPRGSH MGSMERKRWE CPALPQGWER EEVPRRSGLS AGHRDVFYYS PSGKKFRSKP QLARYLGGSM DLSTFDFRTG KMLMSKMNKS RQRVRYDSSN QVKGKPDLNT ALPVRQTASI FKQPVTKITN HPSNKVKSDP QKAVDQPRQL FWEKKLSGLN AFDIAEELVK TMDLPKGLQG VGPGCTDETL LSAIASALHT STMPITGQLS AAVEKNPGVW LNTTQPLCKA FMVTDEDIRK QEELVQQVRK RLEEALMADM LAHVEELARD GEAPLDKACA EDDDEEDEEE EEEEPDPDPE MEHV

purity :

> 85% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol,1mM DTT, 1mM EDTA

concentration :

0.25 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Epigenomics

products description :

Methyl-CpG-binding domain protein 3, also known as MBD3, is a member of the MBD family of transcriptional repressors. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Recombinant human MBD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Nan X., et al. (1998) Nature. 393:386-389. Hendrich B., et al. (1999) Mamm Genome. 10: 906-912.

ncbi gi num :

4505119

ncbi acc num :

NP_003917

ncbi gb acc num :

NM_003926.5

ncbi mol weight :

35.2 kDa (314aa) confirmed by MALDI-TOF

ncbi pathways :

Chromatin Modifying Enzymes Pathway (1127682); Chromatin Organization Pathway (1127681); Gene Expression Pathway (105937); HDACs Deacetylate Histones Pathway (1127684); RNA Polymerase I Promoter Clearance Pathway (106551); RNA Polymerase I Transcription Pathway (106550); RNA Polymerase I Transcription Initiation Pathway (106553); RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway (160948); Signaling Events Mediated By HDAC Class I Pathway (137997)

ncbi summary :

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

uniprot summary :

MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target. Protein type: Transcription, coactivator/corepressor; Gene silencing. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: nucleoplasm; heterochromatin; protein complex; cytoplasm; nuclear chromatin; NuRD complex. Molecular Function: protein binding; nucleosomal DNA binding; DNA binding; methyl-CpG binding. Biological Process: tissue development; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; methylation-dependent chromatin silencing; in utero embryonic development; ATP-dependent chromatin remodeling; histone acetylation; negative regulation of transcription from RNA polymerase II promoter

size1 :

0.05 mg

price1 :

375 USD

size2 :

0.25 mg

price2 :

925

more info or order :

MyBioSource product webpage