product summary
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company name :
MyBioSource
product type :
protein
product name :
GGH, 25-318aa, Human, His tag, E Coli
catalog :
MBS204146
quantity :
0.1 mg
price :
310 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS204146
products type :
Recombinant Protein
products full name :
GGH, 25-318aa, Human, His tag, E Coli
products short name :
GGH
products name syn :
Gamma-glutamyl hydrolase; GH
other names :
gamma-glutamyl hydrolase; Gamma-glutamyl hydrolase; gamma-glutamyl hydrolase; gamma-Glu-X carboxypeptidase; gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase); Conjugase; GH; Gamma-Glu-X carboxypeptidase
products gene name :
GGH
other gene names :
GGH; GGH; GH
uniprot entry name :
GGH_HUMAN
sequence length :
318
sequence :
MGSSHHHHHH SSGLVPRGSH MRPHGDTAKK PIIGILMQKC RNKVMKNYGR YYIAASYVKY LESAGARVVP VRLDLTEKDY EILFKSINGI LFPGGSVDLR RSDYAKVAKI FYNLSIQSFD DGDYFPVWGT CLGFEELSLL ISGECLLTAT DTVDVAMPLN FTGGQLHSRM FQNFPTELLL SLAVEPLTAN FHKWSLSVKN FTMNEKLKKF FNVLTTNTDG KIEFISTMEG YKYPVYGVQW HPEKAPYEWK NLDGISHAPN AVKTAFYLAE FFVNEARKNN HHFKSESEEE KALIYQFSPI YTGNISSFQQ CYIFD
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Metabolism
products description :
Gamma-glutamyl hydrolase, also known as GGH, is a homodimeric protein that catalyzes the cleavage of the gamma-glutamyl chain of folylpoly-gamma-glutamyl substrates and is a central enzyme in folyl and antifolyl poly-gamma-glutamate metabolism. GGH may play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Recombinant human GGH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Rhee M S., et al. (1998) Mol Pharmacol. 53:1040-1046. Galivan J., et al. (1999) Semin Oncol. 26:33-37.
ncbi gi num :
4503987
ncbi acc num :
NP_003869
ncbi gb acc num :
NM_003878.2
ncbi mol weight :
35.9 kDa (315aa) confirmed by MALDI-TOF
ncbi pathways :
Fluoropyrimidine Activity Pathway (198778); Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Glutamate Removal From Folates Pathway (142235); Glutathione-mediated Detoxification Pathway (142435)
ncbi summary :
This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]
uniprot summary :
GGH: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha- glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Belongs to the peptidase C26 family. Protein type: Secreted; EC 3.4.19.9; Secreted, signal peptide; Cofactor and Vitamin Metabolism - folate biosynthesis; Protease. Chromosomal Location of Human Ortholog: 8q12.3. Cellular Component: extracellular space; lysosome; melanosome; cytosol; nucleus. Molecular Function: omega peptidase activity; exopeptidase activity. Biological Process: response to drug; response to ethanol; response to zinc ion; glutamine metabolic process; proteolysis; response to insulin stimulus
size1 :
0.1 mg
price1 :
310 USD
size2 :
0.5 mg
price2 :
720
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
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