catalog number :
MBS204142
products type :
Recombinant Protein
products full name :
Antigen 85A, 43-338aa, Mycobacterium tuberculosis, His tag, Baculovirus
products short name :
Antigen 85A
products name syn :
Antigen 85 complex A; Mycolytransferase 85A; Fibronectin-binding protein A; TB Ab85A; TBab85A
other names :
diacylglycerol acyltransferase/mycolyltransferase Ag85A
sequence :
ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH
purity :
> 90% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Mycobacterium tuberculosis. Tag: His-tag
other info2 :
Expression System: Hi-5
products description :
Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6,6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.
products references :
Elamin AA., et al. (2009) J Microbiol Methods. 79(3):358-63. Jeon BY., et al. (2011) Microbes Infect. 13(3):284-90.
ncbi acc num :
NP_218321.1
ncbi gb acc num :
NC_000962.3
ncbi mol weight :
32.8kDa (305aa)