protein

CRYGD, 1-174aa, Human, His tag, E Coli

MBS204140

0.1 mg

310 USD

Recombinant Protein

CRYGD, 1-174aa, Human, His tag, E Coli

CRYGD

Gamma-crystallin D; CACA; CCA3; CCP; cry-g-D; CRYG4

gamma-crystallin D; Gamma-crystallin D; gamma-crystallin D; gamma crystallin 4; gamma-D-crystallin; gamma-crystallin 4; crystallin, gamma D; Gamma-D-crystallin; Gamma-crystallin 4

CRYGD

CRYGD; CRYGD; CCP; PCC; CACA; CCA3; CRYG4; CTRCT4; cry-g-D; CRYG4

CRGD_HUMAN

174

MGSSHHHHHH SSGLVPRGSH MGKITLYEDR GFQGRHYECS SDHPNLQPYL SRCNSARVDS GCWMLYEQPN YSGLQYFLRR GDYADHQQWM GLSDSVRSCR LIPHSGSHRI RLYEREDYRG QMIEFTEDCS CLQDRFRFNE IHSLNVLEGS WVLYELSNYR GRQYLLMPGD YRRYQDWGAT NARVGSLRRV IDFS

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200mM NaCl

1mg/ml (determined by BCA)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Neuroscience

CRYGD belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in CRYGD are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA). Recombinant human CRYGD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Pande A., et al. (2001) Proc. Natl. Acad. Sci. u.S.A. 98:6116-6120. Plotnikova O.V., et al. (2007) Am. J. Hum. Genet. 81:32-43

13377002

NP_008822

NM_006891.3

22.9kDa (194a), confirmed by MALDI-TOF

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008]

CRYGD: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Defects are the cause of congenital zonular cataract with sutural opacities. Two splice-variant isoforms have been described. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 2q33.3. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; structural constituent of eye lens. Biological Process: lens development in camera-type eye; visual perception. Disease: Cataract 4, Multiple Types

0.1 mg

310 USD

0.5 mg

720