catalog number :
MBS204126
products type :
Recombinant Protein
products full name :
IdhA, 1-329aa, E Coli, His tag, E Coli,
products short name :
IdhA
products name syn :
fermentative D-lactate dehydrogenase; NAD-dependent; ECK1377; hslF; hslI; htpH; JW1375
other names :
fermentative D-lactate dehydrogenase, NAD-dependent; D-lactate dehydrogenase; fermentative D-lactate dehydrogenase, NAD-dependent; Fermentative lactate dehydrogenase
products gene name :
IdhA
other gene names :
ldhA; ldhA; ECK1377; hslF; hslI; htpH; JW1375; hslI; htpH; D-LDH
uniprot entry name :
LDHD_ECOLI
sequence :
MGSSHHHHHH SSGLVPRGSH MGSHMKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP LTPENYHLLN EAAFEQMKNG VMIVNTSRGA LIDSQAAIEA LKNQKIGSLG MDVYENERDL FFEDKSNDVI QDDVFRRLSA CHNVLFTGHQ AFLTAEALTS ISQTTLQNLS NLEKGETCPN ELV
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 100mM NaCl
concentration :
1mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Redox Proteins
products description :
Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E.coli IdhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Atlante, A., et al. (2005) Biochim. Biophys. Acta 1708 (1): 13-22. Martin Engqvist, et al. (2009) J Biol Chem. 284 (September 11): 25026-25037.
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
39.1kDa (353aa), confirmed by MALDI-TOF
ncbi pathways :
Microbial Metabolism In Diverse Environments Pathway 179780!!Pyruvate Metabolism Pathway 1068!!Pyruvate Metabolism Pathway 373!!Mixed Acid Fermentation Pathway 236!!Mixed Acid Fermentation Pathway 139697
ncbi summary :
There are three lactate dehydrogenase enzymes in Escherichia coli which interconvert pyruvate and lactate. [More information is available at EcoCyc: G592].