SHMT1, 1-483aa, Human, His tag, E Coli | MyBioSource MBS204116 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

SHMT1, 1-483aa, Human, His tag, E Coli

catalog :

MBS204116

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204116

products type :

Recombinant Protein

products full name :

SHMT1, 1-483aa, Human, His tag, E Coli

products short name :

SHMT1

products name syn :

Serine hydroxymethyltransferase 1; CSHMT; SHMT

other names :

Serine hydroxymethyltransferase 1 (soluble); Serine hydroxymethyltransferase, cytosolic; serine hydroxymethyltransferase, cytosolic; cytoplasmic serine hydroxymethyltransferase; glycine hydroxymethyltransferase; serine methylase; serine hydroxymethyltransferase 1 (soluble); Glycine hydroxymethyltransferase; Serine methylase

products gene name :

SHMT1

other gene names :

SHMT1; SHMT1; SHMT; CSHMT; SHMT

uniprot entry name :

GLYC_HUMAN

sequence length :

483

sequence :

MGSSHHHHHH SSGLVPRGSH MTMPVNGAHK DADLWSSHDK MLAQPLKDSD VEVYNIIKKE SNRQRVGLEL IASENFASRA VLEALGSCLN NKYSEGYPGQ RYYGGTEFID ELETLCQKRA LQAYKLDPQC WGVNVQPYSG SPANFAVYTA LVEPHGRIMG LDLPDGGHLT HGFMTDKKKI SATSIFFESM PYKVNPDTGY INYDQLEENA RLFHPKLIIA GTSCYSRNLE YARLRKIADE NGAYLMADMA HISGLVAAGV VPSPFEHCHV VTTTTHKTLR GCRAGMIFYR KGVKSVDPKT GKEILYNLES LINSAVFPGL QGGPHNHAIA GVAVALKQAM TLEFKVYQHQ VVANCRALSE ALTELGYKIV TGGSDNHLIL VDLRSKGTDG GRAEKVLEAC SIACNKNTCP GDRSALRPSG LRLGTPALTS RGLLEKDFQK VAHFIHRGIE LTLQIQSDTG VRATLKEFKE RLAGDKYQAA VQALREEVES FASFFPLPGL PDF

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

Serine hydroxymethyltransferase 1, also known as SHMT1, belongs to the SHMT family. This protein is cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5 10-methylene tetrahydrofolate. Also, SHMT1 preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate and inhibits. Recombinant human SHMT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Herbig K., et al. (2002) J Biol Chem. 277:38381-38389. Trivedi V., et al. (2002) J Biol Chem. 277:17161-17169.

ncbi gi num :

14124914

ncbi acc num :

AAH07979

ncbi mol weight :

55.2 kDa (503aa)

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); C1-unit Interconversion, Eukaryotes Pathway (413405); C1-unit Interconversion, Eukaryotes Pathway (468334); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Carnitine Synthesis Pathway (106191); Cyanoamino Acid Metabolism Pathway (82970); Cyanoamino Acid Metabolism Pathway (339); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000)

ncbi summary :

This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

uniprot summary :

SHMT1: Interconversion of serine and glycine. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Methyltransferase; Lyase; EC 2.1.2.1; Other Amino Acids Metabolism - cyanoamino acid; Amino Acid Metabolism - glycine, serine and threonine; Energy Metabolism - methane; Mitochondrial. Chromosomal Location of Human Ortholog: 17p11.2. Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus. Molecular Function: L-allo-threonine aldolase activity; amino acid binding; protein homodimerization activity; glycine hydroxymethyltransferase activity; pyridoxal phosphate binding. Biological Process: purine base biosynthetic process; vitamin metabolic process; folic acid metabolic process; L-serine catabolic process; glycine biosynthetic process from serine; water-soluble vitamin metabolic process; protein tetramerization; carnitine biosynthetic process; protein homotetramerization

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage