protein

HNRNPA1, 1-320aa, Human, His tag, E Coli

MBS204115

0.1 mg

375 USD

Recombinant Protein

HNRNPA1, 1-320aa, Human, His tag, E Coli

HNRNPA1

Heterogeneous nuclear ribonucleoprotein A1; hnRNP A1; hnRNP-A1; HNRPA1; HNRPA1L3

heterogeneous nuclear ribonucleoprotein A1 isoform a; Heterogeneous nuclear ribonucleoprotein A1; heterogeneous nuclear ribonucleoprotein A1; Putative heterogeneous nuclear ribonucleoprotein A1-like 3; helix-destabilizing protein; heterogeneous nuclear ribonucleoprotein A1B protein; heterogeneous nuclear ribonucleoprotein B2 protein; heterogeneous nuclear ribonucleoprotein core protein A1; hnRNP A1-like 3; hnRNP core protein A1; hnRNP core protein A1-like 3; nuclear ribonucleoprotein particle A1 protein; single-strand DNA-binding protein UP1; single-strand RNA-binding protein; heterogeneous nuclear ribonucleoprotein A1; Helix-destabilizing protein; Single-strand RNA-binding protein; hnRNP core protein A1Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed

HNRNPA1

HNRNPA1; HNRNPA1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1; HNRPA1; hnRNP A1

ROA1_HUMAN

320

MGSSHHHHHH SSGLVPRGSH MGSMSKSESP KEPEQLRKLF IGGLSFETTD ESLRSHFEQW GTLTDCVVMR DPNTKRSRGF GFVTYATVEE VDAAMNARPH KVDGRVVEPK RAVSREDSQR PGAHLTVKKI FVGGIKEDTE EHHLRDYFEQ YGKIEVIEIM TDRGSGKKRG FAFVTFDDHD SVDKIVIQKY HTVNGHNCEV RKALSKQEMA SASSSQRGRS GSGNFGGGRG GGFGGNDNFG RGGNFSGRGG FGGSRGGGGY GGSGDGYNGF GNDGSNFGGG GSYNDFGNYN NQSSNFGPMK GGNFGGRSSG PYGGGGQYFA KPRNQGGYGG SSSSSSYGSG RRF

> 85% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

HNRNPA1, also known as heterogeneous nuclear ribonucleoprotein A1, involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. This protein may play a role in HCV RNA replication. Recombinant human HNRNPA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Kim C.S., et al. (2007) J. Virol. 81:3852-3865. Biamonti G., et al. (1989) J. Mol. Biol. 207:491-503

4504445

NP_002127

NM_002136.2

36.6 kDa (343aa) confirmed by MALDI-TOF

Coregulation Of Androgen Receptor Activity Pathway (138085); Gene Expression Pathway (105937); Processing Of Capped Intron-Containing Pre-mRNA Pathway (160950); Spliceosome Pathway (125136); Spliceosome Pathway (124832); MRNA Splicing Pathway (105951); MRNA Splicing - Major Pathway (105952); MRNA Processing Pathway (198843)

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq, Jul 2008]

hnRNP A1: Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with SEPT6. Interacts with HCV NS5B and with the 5 -UTR and 3 -UTR of HCV RNA. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Spliceosome; Nuclear export; RNA-binding; RNA splicing. Chromosomal Location of Human Ortholog: 12q13.1. Cellular Component: nucleoplasm; spliceosome; intermediate filament cytoskeleton; membrane; cytoplasm; ribonucleoprotein complex. Molecular Function: protein binding; single-stranded RNA binding; RNA binding; nucleotide binding; single-stranded DNA binding. Biological Process: nuclear mRNA splicing, via spliceosome; mRNA transport; viral reproduction; RNA export from nucleus; RNA splicing; nuclear export; nuclear import; gene expression; mRNA processing. Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 3; Amyotrophic Lateral Sclerosis 20

0.1 mg

375 USD

0.5 mg

925