product summary
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company name :
MyBioSource
product type :
protein
product name :
DCPS, 1-337aa, Human, His tag, E Coli
catalog :
MBS204108
quantity :
0.05 mg
price :
375 USD
more info or order :
product information
catalog number :
MBS204108
products type :
Recombinant Protein
products full name :
DCPS, 1-337aa, Human, His tag, E Coli
products short name :
DCPS
products name syn :
Scavenger mRNA-decapping enzyme DcpS; DCS1; HINT5
other names :
m7GpppX diphosphatase; m7GpppX diphosphatase; m7GpppX diphosphatase; decapping scavenger enzyme; heat shock-like protein 1; hint-related 7meGMP-directed hydrolase; histidine triad nucleotide-binding protein 5; histidine triad protein member 5; homolog of C. elegans 7meGMP-directed hydrolase dcs-1; mRNA decapping enzyme; scavenger mRNA-decapping enzyme DcpS; decapping enzyme, scavenger; DCS-1; Decapping scavenger enzyme; Hint-related 7meGMP-directed hydrolase; Histidine triad nucleotide-binding protein 5; Histidine triad protein member 5; HINT-5; Scavenger mRNA-decapping enzyme DcpS
products gene name :
DCPS
other gene names :
DCPS; DCPS; DCS1; HSL1; HINT5; HINT-5; HSPC015; DCS1; HINT5; HINT-5
uniprot entry name :
DCPS_HUMAN
sequence length :
337
sequence :
MGSSHHHHHH SSGLVPRGSH MADAAPQLGK RKRELDVEEA HAASTEEKEA GVGNGTCAPV RLPFSGFRLQ KVLRESARDK IIFLHGKVNE ASGDGDGEDA VVILEKTPFQ VEQVAQLLTG SPELQLQFSN DIYSTYHLFP PRQLNDVKTT VVYPATEKHL QKYLRQDLRL IRETGDDYRN ITLPHLESQS LSIQWVYNIL DKKAEADRIV FENPDPSDGF VLIPDLKWNQ QQLDDLYLIA ICHRRGIRSL RDLTPEHLPL LRNILHQGQE AILQRYRMKG DHLRVYLHYL PSYYHLHVHF TALGFEAPGS GVERAHLLAE VIENLECDPR HYQQRTLTFA LRADDPLLKL LQEAQQS
purity :
> 90% by SDS - PAGE
form :
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Enzymes & Proteases
products description :
DCPS, also known as scavenger mRNA-decapping enzyme, is belongs to the HIT family. It is necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. A scavenger decapping enzyme, DcpS was shown to hydrolyze the residual m7GpppN cap structure after the complete 3'-5' degradation of the mRNA by the exosome. DcpS releases m7GMP and is unable to cleave cap structures attached to a long RNA chain. Recombinant human DCPS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
products references :
Liu H., et al. (2002) EMBO J. 21:4699-4708. Kwasnicka D.A., et al. (2003) J. Biol. Chem. 278:39051-39058
ncbi gi num :
7661734
ncbi acc num :
NP_054745
ncbi gb acc num :
NM_014026.4
ncbi mol weight :
40.8 kDa (357aa), confirmed by MALDI-TOF
ncbi pathways :
Deadenylation-dependent MRNA Decay Pathway (160993); Gene Expression Pathway (105937); RNA Degradation Pathway (117291); RNA Degradation Pathway (116127); MRNA Decay By 3' To 5' Exoribonuclease Pathway (160995)
uniprot summary :
DCPS: Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3 5 exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides. Belongs to the HIT family. Protein type: EC 3.6.1.59; Hydrolase; RNA splicing. Chromosomal Location of Human Ortholog: 11q24.2. Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol. Molecular Function: protein binding; m7G(5 )pppN diphosphatase activity; RNA 7-methylguanosine cap binding. Biological Process: deadenylation-dependent decapping; negative regulation of programmed cell death; nuclear mRNA cis splicing, via U2-type spliceosome; gene expression; mRNA catabolic process, deadenylation-dependent decay. Disease: Al-raqad Syndrome
size1 :
0.05 mg
price1 :
375 USD
size2 :
0.25 mg
price2 :
925
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
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