protein

HAO1, 1-370aa, Human, His tag, E Coli

MBS204105

0.1 mg

310 USD

Recombinant Protein

HAO1, 1-370aa, Human, His tag, E Coli

[HAO1]

[Hydroxyacid oxidase 1; GOX (glycolate oxidase); GOX1; HAOX1]

[hydroxyacid oxidase 1; Hydroxyacid oxidase 1; hydroxyacid oxidase 1; hydroxyacid oxidase 1; Glycolate oxidase; GOX]

[HAO1]

[HAO1; HAO1; GOX; GOX1; HAOX1; GOX1; HAOX1; HAOX1; GOX]

E Coli

370

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRML RNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLV RQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIV AKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQ GEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI

> 95% by SDS-PAGE

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.5M NaCl

1.0 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

SDS-Page

Antigen Species: Human. Tag Information: His-tag

Metabolism

HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on twocarbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant human HAO1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Pennati A. and G. Gadda. (2009) J. Biol. Chem. 284:31214; Murray M.S. et al. (2008) Biochemistry, 47:2439

11068137

NP_060015

NM_017545.2

Q9UJM8

45.0 kDa (406aa), confirmed by MALDI-TOF

Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Glyoxylate And Dicarboxylate Metabolism Pathway (83002); Glyoxylate And Dicarboxylate Metabolism Pathway (383); Glyoxylate Metabolism And Glycine Degradation Pathway (1270180); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Amino Acids And Derivatives Pathway (1270158); Peroxisome Pathway (131226); Peroxisome Pathway (131126)

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.

0.1 mg

310 USD

0.5 mg

720