catalog number :
MBS204095
products type :
Recombinant Protein
products full name :
msrA, 1-212aa, E Coli, His tag, E Coli
products short name :
msrA
products name syn :
Peptide methionine sulfoxide reductase A; ECK4215; JW4178; pms; pmsR; peptide-methionine (S)-S-oxide reductase
other names :
methionine sulfoxide reductase A; Peptide methionine sulfoxide reductase MsrA; methionine sulfoxide reductase A; Peptide-methionine (S)-S-oxide reductase; Peptide Met(O) reductase
products gene name :
msrA
other gene names :
msrA; msrA; ECK4215; JW4178; pms; pmsR; pms; Protein-methionine-S-oxide reductase; Peptide Met(O) reductase
uniprot entry name :
MSRA_ECOLI
sequence :
MGSSHHHHHH SSGLVPRGSH MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA
purity :
> 90% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Redox Proteins
products description :
Peptide methionine sulfoxide reductase A, also known msrA, is an enzyme that catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. This protein could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Recombinant E.coli msrA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Kuschel L., et al. (1999) FEBS Letts.1:17-21. Gabbita S., et al. (1999) J Neurochem. 4:1660-1666.
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
25.4 kDa (232aa), confirmed by MALDI-TOF
ncbi summary :
MsrA exhibits methionine sulfoxide reductase activity in vitro (Km of 120 micromolar) . [More information is available at EcoCyc: EG11433].