This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
protein
product name :
nanA, 1-297aa, E Coli, His tag, E Coli
catalog :
MBS204091
quantity :
0.1 mg
price :
310 USD
product information
catalog number :
MBS204091
products type :
Recombinant Protein
products full name :
nanA, 1-297aa, E Coli, His tag, E Coli
products short name :
nanA
products name syn :
N-acetylneuraminate lyase; npl
other names :
N-acetylneuraminate lyase; N-acetylneuraminate lyase; N-acetylneuraminate lyase; N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; NALase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase
products gene name :
nanA
other gene names :
nanA; nanA; ECK3214; JW3194; npl; npl; NAL; Neu5Ac lyase
uniprot entry name :
NANA_ECOLI
sequence length :
297
sequence :
MGSSHHHHHH SSGLVPRGSH MATNLRGVMA ALLTPFDQQQ ALDKASLRRL VQFNIQQGID GLYVGGSTGE AFVQSLSERE QVLEIVAEEA KGKIKLIAHV GCVSTAESQQ LAASAKRYGF DAVSAVTPFY YPFSFEEHCD HYRAIIDSAD GLPMVVYNIP ALSGVKLTLD QINTLVTLPG VGALKQTSGD LYQMEQIRRE HPDLVLYNGY DEIFASGLLA GADGGIGSTY NIMGWRYQGI VKALKEGDIQ TAQKLQTECN KVIDLLIKTG VFRGLKTVLH YMDVVSVPLC RKPFGPVDEK YLPELKALAQ QLMQERG
purity :
> 95% by SDS - PAGE
form :
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Enzymes & Proteases
products description :
NanA, also known as N-acetylneuraminate lyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class I aldolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde. Recombinant E.coli nanA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
products references :
Aisaka K., et al. (1991) Biochem. J. 276:541-546. Izard T., et al. (1994) Structure 2:361-369
ncbi gi num :
16131115
ncbi acc num :
NP_417692
ncbi gb acc num :
NC_000913.3
uniprot acc num :
P0A6L4
ncbi mol weight :
34.7 kDa (317aa) confirmed by MALDI-TOF
ncbi pathways :
Amino Sugar And Nucleotide Sugar Metabolism Pathway 1053!!Amino Sugar And Nucleotide Sugar Metabolism Pathway 350!!N-acetylneuraminate And N-acetylmannosamine Degradation Pathway 150!!N-acetylneuraminate And N-acetylmannosamine Degradation Pathway 139088!!Superpathway Of N-acetylglucosamine, N-acetylmannosamine And N-acetylneuraminate Degradation 147!!Superpathway Of N-acetylglucosamine, N-acetylmannosamine And N-acetylneuraminate Degradation 139078!!Superpathway Of N-acetylneuraminate Degradation 139446
ncbi summary :
N-acetylneuraminate lyase is the first enzyme in the pathway for degradation of sialic acid (N-acetylneuraminate, or NeuNAc) . [More information is available at EcoCyc: EG10637].
size :
0.1 mg
price :
310 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
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