SYT1, 136-382aa, Human, His tag, E Coli | MyBioSource MBS204072 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

SYT1, 136-382aa, Human, His tag, E Coli

catalog :

MBS204072

quantity :

0.01 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204072

products type :

Recombinant Protein

products full name :

SYT1, 136-382aa, Human, His tag, E Coli

products short name :

SYT1

products name syn :

synaptotagmin 1; P65; SVP65; SYT

other names :

synaptotagmin-1 isoform 1; Synaptotagmin-1; synaptotagmin-1; synaptotagmin 1; sytI; synaptotagmin I; Synaptotagmin I; SytI; p65

products gene name :

SYT1

other gene names :

SYT1; SYT1; P65; SYT; SVP65; SVP65; SYT; SytI

uniprot entry name :

SYT1_HUMAN

sequence length :

422

sequence :

MEPKEEEKLG KLQYSLDYDF QNNQLLVGII QAAELPALDM GGTSDPYVKV FLLPDKKKKF ETKVHRKTLN PVFNEQFTFK VPYSELGGKT LVMAVYDFDR FSKHDIIGEF KVPMNTVDFG HVTEEWRDLQ SAEKEEQEKL GDICFSLRYV PTAGKLTVVI LEAKNLKKMD VGGLSDPYVK IHLMQNGKRL KKKKTTIKKN TLNPYYNESF SFEVPFEQIQ KVQVVVTVLD YDKIGKNDAI GKVFVGYNLE HHHHHH

purity :

> 85% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl

concentration :

0.25mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Neuroscience

products description :

Synaptotagmin-1(SYT1) belongs to the synaptotagmin family contains 2 C2 domains. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. SYT1 is the master switch responsible for allowing the human brain to release neurotransmitters. Recombinant human SYT1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Han C., et al. (2009) J. Immunol. 182:2986-2996. Xi, D., et al. (1999) Neuroscience 88: 425-435.

ncbi gi num :

5032139

ncbi acc num :

NP_005630

ncbi gb acc num :

NM_005639.2

ncbi mol weight :

29.5kDa (256aa), confirmed by MALDI-TOF

ncbi pathways :

Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Disease Pathway (530764); Dopamine Neurotransmitter Release Cycle Pathway (106523); Effects Of Botulinum Toxin Pathway (138028); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174); Glutamate Neurotransmitter Release Cycle Pathway (106522); Neuronal System Pathway (106513); Neurotoxicity Of Clostridium Toxins Pathway (105734); Neurotransmitter Release Cycle Pathway (106518); Norepinephrine Neurotransmitter Release Cycle Pathway (106519)

ncbi summary :

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

uniprot summary :

SYT1: an integral membrane protein of synaptic vesicles thought to serve as a Ca(2+) sensor in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse. Binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Protein type: Membrane protein, integral; Vesicle; Calcium-binding. Chromosomal Location of Human Ortholog: 12cen-q21. Cellular Component: presynaptic membrane; SNARE complex; synaptic vesicle; neuron projection; synaptic vesicle membrane; integral to membrane; plasma membrane; excitatory synapse; cell junction. Molecular Function: protein C-terminus binding; identical protein binding; clathrin binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding; calcium-dependent protein binding; calmodulin binding; protein binding; SNARE binding; phosphatidylinositol-4,5-bisphosphate binding; low-density lipoprotein receptor binding; phosphatidylserine binding; syntaxin-1 binding; phosphatidylinositol binding. Biological Process: regulation of synaptic transmission, glutamatergic; neurotransmitter secretion; positive regulation of vesicle fusion; pathogenesis; positive regulation of synaptic transmission; regulation of exocytosis; synaptic transmission; positive regulation of calcium ion-dependent exocytosis; synaptic vesicle exocytosis; vesicle docking; glutamate secretion; detection of calcium ion; protein homooligomerization

size1 :

0.01 mg

price1 :

310 USD

size2 :

0.05 mg

price2 :

720

more info or order :

MyBioSource product webpage