protein

S100A8, 1-89aa, Mouse,His tag, E Coli

MBS204066

0.1 mg

310 USD

Recombinant Protein

S100A8, 1-89aa, Mouse,His tag, E Coli

S100A8

Protein S100-A8; 60B8Ag; AI323541; B8Ag; Caga; CFAg; CP-10; MRP8; p8

protein S100-A8; Protein S100-A8; protein S100-A8; MRP-8; S100 calcium-binding protein A8; calgranulin-A; chemotactic S100 protein; chemotactic cytokine CP-10; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; pro-inflammatory S100 cytokine; S100 calcium binding protein A8 (calgranulin A); Calgranulin-A; Chemotactic cytokine CP-10; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Pro-inflammatory S100 cytokine; S100 calcium-binding protein A8

S100A8

S100a8; S100a8; p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; AI323541; Caga; Mrp8; MRP-8; p8

S10A8_MOUSE

89

MGSSHHHHHH SSGLVPRGSH MPSELEKALS NLIDVYHNYS NIQGNHHALY KNDFKKMVTT ECPQFVQNIN IENLFRELDI NSDNAINFEE FLAMVIKVGV ASHKDSHKE

> 95% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT

1.0 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Mouse. Tag: His-tag

Expression System: E Coli

Cancer

S100A8, also known MRP8, is a member of the S100 family, EF-hand superfamily of calcium binding proteins. This protein is localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Altered expression of this protein is associated with the disease cystic fibrosis. Recombinant mouse S100A8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Kerkhoff C., et al. (1999) Biochim. Biophys. 1448(2):200-11. Nacken W., et al. (2003) Biochim. Biophys. 60(6):569-80.

7305453

NP_038678

NM_013650.2

12.4 kDa (109aa), confirmed by MALDI-TOF

S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family. Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; extracellular region; plasma membrane; nucleus. Molecular Function: antioxidant activity; arachidonic acid binding; RAGE receptor binding; zinc ion binding; metal ion binding; microtubule binding; calcium ion binding. Biological Process: caspase activation; neutrophil chemotaxis; apoptosis; immune system process; regulation of inflammatory response; innate immune response; autophagy; positive regulation of peptide secretion; chemotaxis; inflammatory response; leukocyte migration during inflammatory response; positive regulation of inflammatory response

0.1 mg

310 USD

0.5 mg

720