catalog number :
MBS204054
products type :
Recombinant Protein
products full name :
uFC1, 1-167aa, Human, His tag, E Coli
products short name :
uFC1
products name syn :
ubiquitin-fold modifier-conjugating enzyme 1; HSPC155
other names :
ubiquitin-fold modifier-conjugating enzyme 1; Ubiquitin-fold modifier-conjugating enzyme 1; ubiquitin-fold modifier-conjugating enzyme 1; Ufm1-conjugating enzyme 1; ubiquitin-fold modifier conjugating enzyme 1
products gene name :
uFC1
other gene names :
UFC1; UFC1; HSPC155; Ufm1-conjugating enzyme 1
uniprot entry name :
UFC1_HUMAN
sequence :
MGSSHHHHHH SSGLVPRGSH MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ
purity :
> 90% by SDS - PAGE
form :
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
concentration :
1.0 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
ubiquitination
products description :
uFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1. uFM1 is activated by a novel E1-like enzyme, uBA5, by forming a high-energy thioester bond. Activated uFM1 is then transferred to its cognate E2-like enzyme, uFC1, in a similar thioester linkage. Recombinant human uFC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
products references :
Komatsu M. et al. (2004) EMBO J. 23 :1977-1986. Tatsumi K. et al. (2010) J Biol Chem. 285:5417-5427.
ncbi gb acc num :
NM_016406.3
ncbi mol weight :
21.6 kDa (187aa), confirmed by MALDI-TOF
ncbi summary :
UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Mar 2008]
uniprot summary :
UFC1: E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage. Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily. Protein type: Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 1q23.3. Molecular Function: protein binding