protein

PPCS, 1-311aa, Human, His tag, E Coli

MBS204051

0.1 mg

310 USD

Recombinant Protein

PPCS, 1-311aa, Human, His tag, E Coli

PPCS

Phosphopantothenate--cysteine ligase; COAB; PPC synthetase

phosphopantothenate--cysteine ligase isoform a; Phosphopantothenate--cysteine ligase; phosphopantothenate--cysteine ligase; PPC synthetase; phosphopantothenoylcysteine synthetase; Phosphopantothenoylcysteine synthetase; PPC synthetase

PPCS

PPCS; PPCS; COAB; PPC synthetase

PPCS_HUMAN

311

MGSSHHHHHH SSGLVPRGSH MAEMDPVAEF PQPPGAARWA EVMARFAARL GAQGRRVVLV TSGGTKVPLE ARPVRFLDNF SSGRRGATSA EAFLAAGYGV LFLYRARSAF PYAHRFPPQT WLSALRPSGP ALSGLLSLEA EENALPGFAE ALRSYQEAAA AGTFLAVEFT TLADYLHLLQ AAAQALNPLG PSAMFYLAAA VSDFYVPVSE MPEHKIQSSG GPLQITMKMV PKLLSPLVKD WAPKAFIISF KLETDPAIVI NRARKALEIY QHQVVVANIL ESRQSFVFIV TKDSETKLLL SEEEIEKGVE IEEKIVDNLQ SRHTAFIGDR N

> 95% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol

1.0 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Enzymes & Proteases

PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. Recombinant human PPCS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Manoj N. et al. (2003) Structure. 11 : 927-936. Christina Spry. et al. (2009) J Biol Chem. 284: 24904-24913.

116875844

NP_078940

NM_024664.3

36.1 kDa (331aa) confirmed by MALDI-TOF

Coenzyme A Biosynthesis Pathway (106255); Coenzyme A Biosynthesis, Pantothenate = CoA Pathway (413397); Coenzyme A Biosynthesis, Pantothenate = CoA Pathway (468312); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003)

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008]

PPCS: Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4 - phosphopantothenate to form 4-phosphopantothenoylcysteine. Belongs to the PPC synthetase family. Protein type: Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Ligase; EC 6.3.2.5. Chromosomal Location of Human Ortholog: 1p34.2. Cellular Component: cytosol. Molecular Function: phosphopantothenate--cysteine ligase activity. Biological Process: coenzyme biosynthetic process; coenzyme A biosynthetic process; vitamin metabolic process; pantothenate metabolic process; water-soluble vitamin metabolic process

0.1 mg

310 USD

0.5 mg

720