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company name :
MyBioSource
product type :
protein
product name :
uCHL5, 1-329aa, Human, His tag, E Coli
catalog :
MBS204027
quantity :
0.1 mg
price :
310 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS204027
products type :
Recombinant Protein
products full name :
uCHL5, 1-329aa, Human, His tag, E Coli
products short name :
uCHL5
products name syn :
ubiquitin carboxyl-terminal hydrolase isozyme L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; uCH37; uCH-L5; AD-019; CGI-70; INO80R; INO80 complex subunit R
other names :
ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1; Ubiquitin carboxyl-terminal hydrolase isozyme L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; INO80 complex subunit R; ubiquitin C-terminal hydrolase UCH37; ubiquitin carboxyl-terminal esterase L5; ubiquitin thioesterase L5; ubiquitin carboxyl-terminal hydrolase L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5
products gene name :
uCHL5
other gene names :
UCHL5; UCHL5; UCH37; CGI-70; INO80R; UCH-L5; UCH37; UCH-L5
uniprot entry name :
UCHL5_HUMAN
sequence length :
329
sequence :
MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
purity :
> 90% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200mM NaCl, 0.1mM PMSF, 2mM EDTA
concentration :
0.5mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli. Bioactivity: Specific activity: > 500 pmole/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C. Assay: 1. Prepare a 100ul of recombinant uCH-L5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37Cfor 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) . 2. Add 50ul of 1uM ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - ubiquitin-AMC (Enzo, Cat.No, BML-SE211) . - 96 Well Polystyrene Microplate, black (greiner bio-one, Cat.No, 655077) . - Fluorescent plate reader (PerkinElmer, VICTOR X3)
products categories :
ubiquitination
products description :
ubiquitin carboxyl-terminal hydrolase isozyme L5 (uCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human uCHL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Yao T., et al. (2006) Nat. Cell Biol. 8:994-1002. Nishio K, et al.(2009) Biochem Biophys Res Commun. 390(3):855-60.
ncbi gi num :
7706753
ncbi acc num :
NP_057068
ncbi gb acc num :
NM_015984.3
ncbi mol weight :
39.7kDa (349aa), confirmed by MALDI-TOF
ncbi pathways :
Disease Pathway 530764!!Downregulation Of TGF-beta Receptor Signaling Pathway 645293!!Loss Of Function Of SMAD2/3 In Cancer Pathway 906019!!Loss Of Function Of SMAD4 In Cancer Pathway 906017!!Loss Of Function Of TGFBR1 In Cancer Pathway 906025!!Loss Of Function Of TGFBR2 In Cancer Pathway 906022!!SMAD2/3 MH2 Domain Mutants In Cancer Pathway 906021!!SMAD2/3 Phosphorylation Motif Mutants In Cancer Pathway 906020!!SMAD4 MH2 Domain Mutants In Cancer Pathway 906018!!Signal Transduction Pathway 477114
size :
0.1 mg
price :
310 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
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