catalog number :
MBS204014
products type :
Recombinant Protein
products full name :
BDH2, 1-245aa, Human, His tag, E Coli
products short name :
[BDH2]
products name syn :
[3-hydroxybutyrate dehydrogenase type 2; DHRS6; EFA6R; FLJ13261; PRO20933; SDR15C1; uCPA-OR; uNQ6308]
other names :
[3-hydroxybutyrate dehydrogenase type 2; 3-hydroxybutyrate dehydrogenase type 2; 3-hydroxybutyrate dehydrogenase type 2; 3-hydroxybutyrate dehydrogenase 2; Dehydrogenase/reductase SDR family member 6; Oxidoreductase UCPA; R-beta-hydroxybutyrate dehydrogenase; Short chain dehydrogenase/reductase family 15C member 1]
products gene name :
[BDH2]
other gene names :
[BDH2; BDH2; DHRS6; EFA6R; SDR15C1; UCPA-OR; UNQ6308; PRO20933; DHRS6; SDR15C1]
sequence positions :
[1-245aa]
sequence :
MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAN EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL
purity :
> 95% by SDS-PAGE
form :
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
concentration :
1.0 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
image1 heading :
SDS-Page
other info1 :
Antigen Species: Human. Tag Information: His-tag
products categories :
Metabolism
products description :
BDH2, also known as DHRS6, belongs to the short-chain dehydrogenases/reductases (SDR) family. This protein may play an important role in the peripheral utilization of 3-hydroxybutyrate. The cytoplasmic localization with its high ratio of oxidized NAD+, the NAD+ dependence and the kinetic parameters of BDH2 make it suitable to convert high levels of circulating 3-hydroxybutyrate into acetoacetate. Recombinant human BDH2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
products references :
Guo K., et al. (2006). J. Biol. Chem., 281: 10291-10297; Ito K., et al. (2006). J. Mol. Biol., 355: 722-733.
ncbi gb acc num :
NM_020139.3
ncbi mol weight :
28.8 kDa (265aa), confirmed by MALDI-TOF
ncbi pathways :
Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (1270010); Ketone Body Metabolism Pathway (1270026); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Synthesis And Degradation Of Ketone Bodies Pathway (82936); Synthesis And Degradation Of Ketone Bodies Pathway (297); Synthesis Of Ketone Bodies Pathway (1270027)
uniprot summary :
Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase ().