catalog number :
MBS204001
products type :
Recombinant Protein
products full name :
RBKS, 1-322aa, Human, His tag, E Coli
products short name :
RBKS
products name syn :
Ribokinase; RBSK
other names :
ribokinase isoform 1; Ribokinase; ribokinase; ribokinase
products gene name :
RBKS
other gene names :
RBKS; RBKS; RBSK; RBSK
uniprot entry name :
RBSK_HUMAN
sequence :
MGSSHHHHHH SSGLVPRGSH MAASGEPQRQ WQEEVAAVVV VGSCMTDLVS LTSRLPKTGE TIHGHKFFIG FGGKGANQCV QAARLGAMTS MVCKVGKDSF GNDYIENLKQ NDISTEFTYQ TKDAATGTAS IIVNNEGQNI IVIVAGANLL LNTEDLRAAA NVISRAKVMV CQLEITPATS LEALTMARRS GVKTLFNPAP AIADLDPQFY TLSDVFCCNE SEAEILTGLT VGSAADAGEA ALVLLKRGCQ VVIITLGAEG CVVLSQTEPE PKHIPTEKVK AVDTTGAGDS FVGALAFYLA YYPNLSLEDM LNRSNFIAAV SVQAAGTQSS YPYKKDLPLT LF
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing,10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Metabolism
products description :
Ribokinase, also known as RBKS, belongs to the pfkB family of carbohydrate kinases. It phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism. Recombinant human RBKS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Sigrell J A., et al. (1999) J Mol Biol. 290:1009-1018. Park J., et al. (2007) FEBS Lett. 581:3211-3216.
ncbi gb acc num :
NM_022128.2
ncbi mol weight :
36.3 kDa (342aa), confirmed by MALDI-TOF
ncbi pathways :
Pentose Phosphate Pathway (82928); Pentose Phosphate Pathway (289)
ncbi summary :
This gene encodes a member of the carbohydrate kinase PfkB family. The encoded protein phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
uniprot summary :
RBKS: The ribokinase encoded by this gene belongs to the pfkB family of carbohydrate kinases. It phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism. [provided by RefSeq, Jul 2008]. Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Transferase; EC 2.7.1.15. Chromosomal Location of Human Ortholog: 2p23.3. Molecular Function: ribokinase activity; ATP binding. Biological Process: carbohydrate phosphorylation; D-ribose catabolic process