protein

ROBLD3, 1-125aa, Human, His tag, E Coli

MBS203981

0.1 mg

375 USD

Recombinant Protein

ROBLD3, 1-125aa, Human, His tag, E Coli

ROBLD3

Mitogen-activated protein-binding protein-interacting protein; ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; p14

ragulator complex protein LAMTOR2 isoform 1; Ragulator complex protein LAMTOR2; ragulator complex protein LAMTOR2; MAPBP-interacting protein; MAPKSP1 adaptor protein; endosomal adaptor protein p14; late endosomal/lysosomal Mp1-interacting protein; late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; mitogen-activated protein-binding protein-interacting protein (MAPBPIP); roadblock domain containing 3; roadblock domain-containing protein 3; late endosomal/lysosomal adaptor, MAPK and MTOR activator 2; Endosomal adaptor protein p14; Late endosomal/lysosomal Mp1-interacting protein; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; Mitogen-activated protein-binding protein-interacting protein; MAPBP-interacting protein; Roadblock domain-containing protein 3

ROBLD3

LAMTOR2; LAMTOR2; p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2; MAPBPIP; ROBLD3; MAPBP-interacting protein

LTOR2_HUMAN

125

MGSSHHHHHH SSGLVPRGSH MGSHMLRPKA LTQVLSQANT GGVQSTLLLN NEGSLLAYSG YGDTDARVTA AIASNIWAAY DRNGNQAFNE DNLKFILMDC MEGRVAITRV ANLLLCMYAK ETVGFGMLKA KAQALVQYLE EPLTQVAAS

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M Nacl, 2mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

ROBLD3, also known as MAPBPIP, belongs to the GAMAD family. It is an adapter protein that enhances the efficiency of the MAP kinase cascade and facilitates the activation of MAPK2. ROBLD3 compels the recruitment of MP1 to late endosomes where they form a very stable heterodimeric complex required for ERK activation on endosomes. Recombinant human ROBLD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Sancak Y., et al. (2010) Cell. 141(2):290-303. Waters S., et al. (2009) FEBS Lett. 583(12):1846-52.

7661728

NP_054736

NM_014017.3

16.0 kDa (149aa), confirmed by MALDI-TOF

The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

LAMTOR2: Adapter protein that enhances the efficiency of the MAP kinase cascade. Facilitates the activation of MAPKAPK2 . Heterodimer with MAP2K1IP1. Interacts with ERK and MEK1. Defects in ROBLD3 are the cause of immunodeficiency due to defect in MAPBP-interacting protein. This form of primary immunodeficiency syndrome includes congenital neutropenia, partial albinism, short stature and B-cell and cytotoxic T-cell deficiency. Two alternatively spliced isoforms of the human protein have been described. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: lysosomal membrane; late endosome. Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; protein complex scaffold. Biological Process: activation of MAPKK activity; cell growth; positive regulation of TOR signaling pathway; positive regulation of GTPase activity. Disease: Immunodeficiency Due To Defect In Mapbp-interacting Protein

0.1 mg

375 USD

0.5 mg

925