protein

NCALD, 1-193aa, Human, His tag, E Coli

MBS203973

0.1 mg

310 USD

Recombinant Protein

NCALD, 1-193aa, Human, His tag, E Coli

NCALD

Neurocalcin-delta; MGC33870; MGC74858; Neurocalcin delta

neurocalcin-delta; Neurocalcin-delta; neurocalcin-delta; neurocalcin delta

NCALD

NCALD; NCALD

NCALD_HUMAN

193

MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 100mM NaCl

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Neuroscience

Neurocalcin-delta, also known NCALD, is a member of the neuronal calcium sensor (NCS) family. NCALD is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. This protein is thought to be a regulator of signal transduction by G protein-coupled receptor. Recombinant human NCALD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Burqoyne RD., et al. (2007) Nat Rev Neurosci. 8(3):182-93. Burqoyne RD., et al. (2004) Trends Neurosci. 27(4): 203-9

98985795

NP_001035720

NM_001040630.1

24.4 kDa (213aa), confirmed by MALDI-TOF

Activation Of Ca-permeable Kainate Receptor Pathway (161046); Activation Of Kainate Receptors Upon Glutamate Binding Pathway (161043); Ionotropic Activity Of Kainate Receptors Pathway (161044); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Transmission Across Chemical Synapses Pathway (106516)

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

NCALD: May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions. Belongs to the recoverin family. Chromosomal Location of Human Ortholog: 8q22.2. Cellular Component: clathrin coat of trans-Golgi network vesicle; intracellular; cytosol. Molecular Function: tubulin binding; protein binding; clathrin binding; calcium ion binding; actin binding; alpha-tubulin binding. Biological Process: vesicle-mediated transport; synaptic transmission; regulation of systemic arterial blood pressure; calcium-mediated signaling

0.1 mg

310 USD

0.5 mg

720