protein

RQCD1, 1-299aa, Human, His tag, E Coli

MBS203971

0.1 mg

310 USD

Recombinant Protein

RQCD1, 1-299aa, Human, His tag, E Coli

RQCD1

Cell differentiation protein RCD1 homolog; CNOT9; CT129; RCD1

cell differentiation protein RCD1 homolog isoform 2; Cell differentiation protein RCD1 homolog; cell differentiation protein RCD1 homolog; CCR4-NOT transcription complex subunit 9; cancer/testis antigen 129; protein involved in sexual development; RCD1 required for cell differentiation1 homolog (S. pombe); CCR4-NOT transcription complex subunit 9

RQCD1

RQCD1; RQCD1; RCD1; CNOT9; CT129; RCD-1; CNOT9; RCD1; Rcd-1

RCD1_HUMAN

299

MGSSHHHHHH SSGLVPRGSH MHSLATAAPV PTTLAQVDRE KIYQWINELS SPETRENALL ELSKKRESVP DLAPMLWHSF GTIAALLQEI VNIYPSINPP TLTAHQSNRV CNALALLQCV ASHPETRSAF LAAHIPLFLY PFLHTVSKTR PFEYLRLTSL GVIGALVKTD EQEVINFLLT TEIIPLCLRI MESGSELSKT VATFILQKIL LDDTGLAYIC QTYERFSHVA MILGKMVLQL SKEPSARLLK HVVRCYLRLS DNPRAREALR QCLPDQLKDT TFAQVLKDDT TTKRWLAQLV KNLQEGQVTD PRGIPLPPQ

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Transcription & Translation

RQCD1, also known as RCD1, is a novel transcriptional cofactor that mediates retinoic acid-induced cell differentiation. It down-regulates MYB- and JuN-dependent transcription and may play a role in cell differentiation. RQCD1 also plays a role in retinoic acid-controlled lung development. Recombinant human RQCD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Garapaty S., et al. (2008) J Biol Chem. 283(11):6806-16. Hiroi N., et al. (2002) EMBO J. 21(19):5235-44.

4885579

NP_005435

NM_005444.2

35.7 kDa (319aa) confirmed by MALDI-TOF

Deadenylation Of MRNA Pathway (160994); Deadenylation-dependent MRNA Decay Pathway (160993); Gene Expression Pathway (105937); RNA Degradation Pathway (117291); RNA Degradation Pathway (116127)

This gene encodes a member of the highly conserved RCD1 protein family. The encoded protein is a transcriptional cofactor and a core protein of the CCR4-NOT complex. It may be involved in signal transduction as well as retinoic acid-regulated cell differentiation and development. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2012]

RQCD1: Transcription factor that down-regulates MYB- and JUN- dependent transcription. May play a role in cell differentiation. Can bind oligonucleotides, such as poly-G, poly-C or poly-T (in vitro), but the physiological relevance of this is not certain. Does not bind poly-A. Enhances ligand-dependent transcriptional activity of nuclear hormone receptors, including RARA, expect ESR1-mediated transcription that is not only slightly increased, if at all. Belongs to the RCD1 family. Protein type: Cancer Testis Antigen (CTA). Chromosomal Location of Human Ortholog: 2q35. Cellular Component: membrane; nucleus; cytosol; CCR4-NOT complex. Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity. Biological Process: regulation of translation; poly(A) tail shortening; regulation of transcription, DNA-dependent; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; RNA-mediated gene silencing; gene expression; sex differentiation; mRNA catabolic process, deadenylation-dependent decay; negative regulation of estrogen receptor signaling pathway

0.1 mg

310 USD

0.5 mg

720