protein

uBE2K, 1-200aa, Human, His tag, E Coli

MBS203958

0.05 mg

375 USD

Recombinant Protein

uBE2K, 1-200aa, Human, His tag, E Coli

uBE2K

ubiquitin-conjugating enzyme E2 K isoform 1; HIP2; HYPG; LIG

ubiquitin-conjugating enzyme E2 K isoform 1; Ubiquitin-conjugating enzyme E2 K; ubiquitin-conjugating enzyme E2 K; E2(25K); HIP-2; huntingtin interacting protein 2; huntingtin-interacting protein 2; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-protein ligase; ubiquitin-conjugating enzyme E2K; Huntingtin-interacting protein 2; HIP-2; Ubiquitin carrier protein; Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25K; Ubiquitin-protein ligase

uBE2K

UBE2K; UBE2K; LIG; HIP2; HYPG; UBC1; E2-25K; HIP2; LIG; HIP-2; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25K

UBE2K_HUMAN

200

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 50mM NaCl

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

ubiquitination

ubiquitin-conjugating enzyme E2 K isoform 1, also known as uBE2K, is a member of the ubiquitin-conjugating enzymes family. These enzymes participate in a variety of cellular processes, including selective protein degradation, DNA repair, cell cycle control, and sporulation. In this mechanism, the ATP-coupled activation and subsequent ligation of ubiquitin are catalyzed by separate enzymes functionally linked by ubiquitin carrier protein uBC1. Also, uBE2K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-beta, huntingtin, and MHC-heavy chain proteins. Recombinant human uBE2K protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Chen Z., et al. (1990) J Biol Chem. 264:21835-21742. Pichler A., et al. (2005) Nat Struct Mol. 12:264-269.

4885417

NP_005330

NM_005339.4

26.5 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386); Innate Immune System Pathway (106387); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (187102); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (187097); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

UBE2K: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of Lys-48 -linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein- dependent degradation of RB1. Interacts with RNF138/NARF. Interacts with BRCA1. By aggregated low-density lipoprotein. Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.3.2.19; Ligase; Ubiquitin ligase; Ubiquitin conjugating system. Cellular Component: filopodium tip; intermediate filament cytoskeleton; cytoplasm; nucleus; actin cytoskeleton. Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; nucleotide binding; ATP binding; ligase activity. Biological Process: protein polyubiquitination; regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination

0.05 mg

375 USD

0.25 mg

925