catalog number :
MBS203941
products type :
Recombinant Protein
products full name :
eFIMC, 37-241aa, E Coli, His tag, E Coli
products short name :
eFIMC
products name syn :
Periplasmic chaperone; required for type 1 fimbriae; ECK4307; JW4279
other names :
periplasmic chaperone; Chaperone protein FimC; periplasmic chaperone
products gene name :
eFIMC
other gene names :
fimC; fimC; ECK4307; JW4279
uniprot entry name :
FIMC_ECOLI
sequence :
MGSSHHHHHH SSGLVPRGSH MGVALGATRV IYPAGQKQEQ LAVTNNDENS TYLIQSWVEN ADGVKDGRFI VTPPLFAMKG KKENTLRILD ATNNQLPQDR ESLFWMNVKA IPSMDKSKLT ENTLQLAIIS RIKLYYRPAK LALPPDQAAE KLRFRRSANS LTLINPTPYY LTVTELNAGT RVLENALVPP MGESTVKLPS DAGSNITYRT INDYGALTPK MTGVME
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT.
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Heat Shock Proteins
products description :
The 23 kDa two-domain periplasmic chaperone FIMC from Escherichia coli is required for the assembly of type-1 pili, which are filamentous, highly oligomeric protein complexes anchored to the outer bacterial membrane that mediate adhesion of pathogenic E. coli strains to host cell surfaces and persist in macrophages. Recombinant E. coli FIMC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Hermanns u., et al. (2000) Biochemistry. 39(38):11564-70.
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
25 kDa (226aa), confirmed by MALDI-TOF
ncbi summary :
FimC has Ig-like domains: pfam PF00345 aligns to N-terminal residues 37-157, and pfam PF02753 aligns to C-terminal residues 161-237. [More information is available at EcoGene: EG10310]. FimC is a member of the periplasmic chaperone family which functions in the chaperone-usher pathway and is indispensable in the biogenesis of the type 1 pilus fiber of Escherichia coli . [More information is available at EcoCyc: EG10310].