MAPRE1, 1-268aa, Human, His tag, E Coli | MyBioSource MBS203939 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MAPRE1, 1-268aa, Human, His tag, E Coli

catalog :

MBS203939

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203939

products type :

Recombinant Protein

products full name :

MAPRE1, 1-268aa, Human, His tag, E Coli

products short name :

MAPRE1

products name syn :

Microtubule-associated protein RP/EB family member 1; EB1; MGC117374; MGC129946

other names :

microtubule-associated protein RP/EB family member 1; Microtubule-associated protein RP/EB family member 1; microtubule-associated protein RP/EB family member 1; APC-binding protein EB1; adenomatous polyposis coli-binding protein EB1; end-binding protein 1; microtubule-associated protein, RP/EB family, member 1; APC-binding protein EB1; End-binding protein 1; EB1

products gene name :

MAPRE1

other gene names :

MAPRE1; MAPRE1; EB1; EB1

uniprot entry name :

MARE1_HUMAN

sequence length :

268

sequence :

MGSSHHHHHH SSGLVPRGSH MAVNVYSTSV TSDNLSRHDM LAWINESLQL NLTKIEQLCS GAAYCQFMDM LFPGSIALKK VKFQAKLEHE YIQNFKILQA GFKRMGVDKI IPVDKLVKGK FQDNFEFVQW FKKFFDANYD GKDYDPVAAR QGQETAVAPS LVAPALNKPK KPLTSSSAAP QRPISTQRTA AAPKAGPGVV RKNPGVGNGD DEAAELMQQV NVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD EGFVIPDEGG PQEEQEEY

purity :

> 90 % by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 2mM DTT, 0.1M NaCl.

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Cytoskeleton

products description :

MAPRE1, also known as EB1, belongs to the intermediate/early gene family. This protein first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. It localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. MAPRE1 is involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. Recombinant human MAPRE1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Zhang T., et al. (2009) J Cell Sci. 122(Pt 9):1401-9. Tirnauer JS., et al. (2002) Mol Biol Cell. 13(10):3614-26.

ncbi gi num :

6912494

ncbi acc num :

NP_036457

ncbi gb acc num :

NM_012325.2

ncbi mol weight :

32.2 kDa (288aa) confirmed by MALDI-TOF

ncbi pathways :

Anchoring Of The Basal Body To The Plasma Membrane Pathway (1127503); Assembly Of The Primary Cilium Pathway (1127502); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Centrosome Maturation Pathway (105807); G2/M Transition Pathway (105801); Loss Of Nlp From Mitotic Centrosomes Pathway (105811); Loss Of Proteins Required For Interphase Microtubule Organizationfrom The Centrosome Pathway (105810); M Phase Pathway (105812); Mitotic Anaphase Pathway (105818)

ncbi summary :

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]

uniprot summary :

EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 20q11.1-q11.23. Cellular Component: Golgi apparatus; microtubule; centrosome; cell projection membrane; cytoplasmic microtubule; cytoplasm; cortical microtubule cytoskeleton; spindle; cytosol. Molecular Function: protein C-terminus binding; microtubule plus-end binding; protein binding. Biological Process: cell proliferation; mitosis; cell division; organelle organization and biogenesis; negative regulation of microtubule polymerization; mitotic cell cycle; G2/M transition of mitotic cell cycle

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage