protein

TSN, 1-228aa, Human, E Coli

MBS203933

0.1 mg

375 USD

Recombinant Protein

TSN, 1-228aa, Human, E Coli

TSN

Translin; BCLF-1; RCHF1; REHF-1; TBRBP; TRSLN

translin isoform 1; Translin; translin; component 3 of promoter of RISC; recombination hotspot associated factor; recombination hotspot-binding protein; testis brain-RNA binding protein; translin; Component 3 of promoter of RISC; C3PO

TSN

TSN; TSN; C3PO; RCHF1; TBRBP; TRSLN; BCLF-1; REHF-1; C3PO

TSN_HUMAN

228

MSVSEIFVEL QGFLAAEQDI REEIRKVVQS LEQTAREILT LLQGVHQGAG FQDIPKRCLK AREHFGTVKT HLTSLKTKFP AEQYYRFHEH WRFVLQRLVF LAAFVVYLET ETLVTREAVT EILGIEPDRE KGFHLDVEDY LSGVLILASE LSRLSVNSVT AGDYSRPLHI STFINELDSG FRLLNLKNDS LRKRYDGLKY DVKKVEEVVY DLSIRGFNKE TAAACVEK

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol. 100mM NaCl

1 mg/ml (determined by Bradford assay

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli

Transportation

Translin, also known TSN, is a DNA and RNA binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin forms a ring-shaped structure, which is responsible for DNA binding, and also contains a leucine zipper motif, which is thought to enable translin to form dimers. Translin exports specific mRNAs out of the nucleus, supported by its localization in both the nuclei and cytoplasm of neurons, and regulates their translation. Recombinant human Translin protein, was expressed in E.coli and purified by using conventional chromatography techniques.

Gu W., et al. (1998) Mol Reprod Dev. 49:219-228. Aoki K., et al. (1999) FEBS Lett. 443:363-366.

4759270

NP_004613

NM_004622.2

26.1kDa (228aa) confirmed by MALDI-TOF

Gene Expression Pathway (105937); Regulatory RNA Pathways (106334); Small Interfering RNA (siRNA) Biogenesis Pathway (576277)

This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. This protein encoded by this gene, when complexed with translin-associated protein X, also forms a Mg ion-dependent endoribonuclease that promotes RNA-induced silencing complex (RISC) activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

TSN: DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots. Belongs to the translin family. Protein type: Nuclear receptor co-regulator; DNA-binding; EC 3.1.-.-. Chromosomal Location of Human Ortholog: 2q21.1. Cellular Component: cytoplasm; nucleus; cytosol. Molecular Function: mRNA binding; endoribonuclease activity; protein binding; DNA binding; sequence-specific DNA binding; protein complex binding; single-stranded DNA binding. Biological Process: gene expression; DNA recombination

0.1 mg

375 USD

0.5 mg

925