BLMH, 1-455aa, Human, His tag, E Coli | MyBioSource MBS203929 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

BLMH, 1-455aa, Human, His tag, E Coli

catalog :

MBS203929

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203929

products type :

Recombinant Protein

products full name :

BLMH, 1-455aa, Human, His tag, E Coli

products short name :

BLMH

products name syn :

Bleomycin hydrolase; BH; BMH

other names :

bleomycin hydrolase; Bleomycin hydrolase; bleomycin hydrolase; BLM hydrolase; bleomycin hydrolase

products gene name :

BLMH

other gene names :

BLMH; BLMH; BH; BMH; BH; BLM hydrolase; BMH

uniprot entry name :

BLMH_HUMAN

sequence length :

455

sequence :

MGSSHHHHHH SSGLVPRGSH MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM GALAE

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: Specific activity: > 1,000 pmole/min/ug. Measured by the hydrolysis of Met-AMC at pH 7.5, at 37 degree C. Assay: 1. Prepare a 50ul of recombinant BLMH protein with various concentrations (0.06 ug, 0.03 ug) in assay buffer and equilibrate to 37C for 30 minutes. (Assay buffer: 50mM Tris-HCl, 5 mM EDTA, 10 mM DTT, pH 7.5.) . 2. Add 50ul of 2mM Met-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Met-AMC (Enzo, Cat.No, BML-P236-0025) . - 96 Well Polystyrene Microplate, black (greiner bio-one, Cat.No, 655077) . - Fluorescent plate reader (PerkinElmer, VICTOR X3) .

products categories :

Proteases & Inhibitors

products description :

BLMH is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but it protects normal and malignant cells from the glycopeptide antitumor drug BLM. It catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and also shows general aminopeptidase activity. Recombinant human BLMH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Lazo JS.,et al. (2007) Neuroscience. 146(3):890-900. Abernethy J.,et al (1991) Proc Natl Acad Sci u S A. 96(8):4680-5.

ncbi gi num :

4557367

ncbi acc num :

NP_000377

ncbi gb acc num :

NM_000386.3

ncbi mol weight :

54.7 kDa(475aa), confirmed by MALDI-TOF

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386)

ncbi summary :

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]

uniprot summary :

BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family. Protein type: Protease; EC 3.4.22.40. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; carboxypeptidase activity; cysteine-type endopeptidase activity; aminopeptidase activity; cysteine-type peptidase activity. Biological Process: response to drug; antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; response to toxin; proteolysis. Disease: Alzheimer Disease

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage