product summary
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company name :
MyBioSource
product type :
protein
product name :
Beta-lactamase, 20-377aa, E Coli, His tag, E Coli
catalog :
MBS203877
quantity :
0.1 mg
price :
310 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS203877
products type :
Recombinant Protein
products full name :
Beta-lactamase, 20-377aa, E Coli, His tag, E Coli
products short name :
Beta-lactamase
products name syn :
ampC; ampA; Cephalosporinase; D-alanine carboxypeptidase
other names :
penicillin-binding protein; beta-lactamase, intrinsically weak; Beta-lactamase; penicillin-binding protein; beta-lactamase, intrinsically weak; Cephalosporinase
other gene names :
ampC; ampC; ampA; ECK4146; JW4111; ampA
uniprot entry name :
AMPC_ECOLI
sequence length :
377
sequence :
MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ
purity :
> 95% by SDS - PAGE
form :
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Metabolism
products description :
AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E.coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
products references :
Jaurin B., et al. (1981) Proc. Natl. Acad. Sci. u.S.A. 78:4897-4901. Morandi F, et al. (2003) J AM Chem Soc. 125(3):685-95
ncbi gi num :
16131975
ncbi acc num :
NP_418574
ncbi gb acc num :
NC_000913.3
uniprot acc num :
P00811
ncbi mol weight :
41.8 kDa (379aa) confirmed by MALDI-TOF
ncbi pathways :
Biosynthesis Of Secondary Metabolites Pathway (148665); Two-component System Pathway (1114); Two-component System Pathway (437); Beta-Lactam Resistance Pathway (1060015)
ncbi summary :
False positive lipoprotein prediction. [More information is available at EcoGene: EG10040]. The ampC -lactamase gene encodes a serine cephalosporinase . [More information is available at EcoCyc: EG10040].
size1 :
0.1 mg
price1 :
310 USD
size2 :
0.5 mg
price2 :
720
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

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