protein

A2LD1, 1-153aa, Human, His tag, E Coli

MBS203874

0.05 mg

375 USD

Recombinant Protein

A2LD1, 1-153aa, Human, His tag, E Coli

A2LD1

Gamma-glutamylaminecyclotransferase; GGACT

gamma-glutamylaminecyclotransferase; Gamma-glutamylaminecyclotransferase; gamma-glutamylaminecyclotransferase; AIG2-like domain 1; AIG2-like domain-containing protein 1; gamma-glutamylamine cyclotransferase; AIG2-like domain-containing protein 1; Gamma-glutamylamine cyclotransferase

A2LD1

GGACT; GGACT; A2LD1; A2LD1; GGACT

GGACT_HUMAN

153

MGSSHHHHHH SSGLVPRGSH MALVFVYGTL KRGQPNHRVL RDGAHGSAAF RARGRTLEPY PLVIAGEHNI PWLLHLPGSG RLVEGEVYAV DERMLRFLDD FESCPALYQR TVLRVQLLED RAPGAEEPPA PTAVQCFVYS RATFPPEWAQ LPHHDSYDSE GPHGLRYNPR ENR

> 95% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl

1.0 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Enzymes & Proteases

A2LD1, also known as gamma-glutamylaminecyclotransferase, is an enzyme that converts gamma-glutamylamines to free amines and 5-oxoproline. It shows high activity toward gamma-glutamyl-epsilon-lysine, derived from the breakdown of fibrin and other proteins cross-linked by transglutaminases. This protein adopts the newly identified cyclotransferase fold, observed in gamma-glutamylcyclotransferase, an enzyme with activity toward gamma-glutamyl-alpha-amino acids. Recombinant human A2LD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Oakley AJ,., et al. (2010), J Biol Chem., 285:9642-8. Danson JW., et al. (2002), Anal Biochem., 303:120-30.

34147453

NP_149101

NM_033110.2

19.4 kDa (173aa) confirmed by MALDI-TOF

The protein encoded by this gene aids in the proteolytic degradation of crosslinked fibrin by breaking down isodipeptide L-gamma-glutamyl-L-epsilon-lysine, a byproduct of fibrin degradation. The reaction catalyzed by the encoded gamma-glutamylaminecyclotransferase produces 5-oxo-L-proline and a free alkylamine. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Aug 2010]

A2LD1: Contributes to degradation of proteins cross-linked by transglutaminases. Degrades the cross-link between a lysine and a glutamic acid residue from two proteins that have been cross- linked by transglutaminases. Catalyzes the formation of 5- oxoproline from L-gamma-glutamyl-L-epsilon-lysine. Inactive with L-gamma-glutamyl-alpha-amino acid substrates such as L-gamma- glutamyl-L-alpha-cysteine and L-gamma-glutamyl-L-alpha-alanine. Belongs to the gamma-glutamylcyclotransferase family. Protein type: EC 2.3.2.4; Transferase. Chromosomal Location of Human Ortholog: 13q32.3. Molecular Function: gamma-glutamylcyclotransferase activity. Biological Process: amino acid derivative catabolic process

0.05 mg

375 USD

0.25 mg

925