RPA2, 1-270aa, Human, His tag, E Coli | MyBioSource MBS203846 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

RPA2, 1-270aa, Human, His tag, E Coli

catalog :

MBS203846

quantity :

0.05 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203846

products type :

Recombinant Protein

products full name :

RPA2, 1-270aa, Human, His tag, E Coli

products short name :

RPA2

products name syn :

Replication protein A 32 kDa subunit; REPA2; RPA32; RPA32; RP-A p32; RP-A p34

other names :

replication protein A 32 kDa subunit isoform 1; Replication protein A 32 kDa subunit; replication protein A 32 kDa subunit; RF-A protein 2; replication factor A protein 2; replication protein A 34 kDa subunit; replication protein A2, 32kDa; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34

products gene name :

RPA2

other gene names :

RPA2; RPA2; REPA2; RPA32; RP-A p32; RP-A p34; REPA2; RPA32; RPA34; RP-A p32; RF-A protein 2; RP-A p34

uniprot entry name :

RFA2_HUMAN

sequence length :

270

sequence :

MGSSHHHHHH SSGLVPRGSH MGSMWNSGFE SYGSSSYGGA GGYTQSPGGF GSPAPSQAEK KSRARAQHIV PCTISQLLSA TLVDEVFRIG NVEISQVTIV GIIRHAEKAP TNIVYKIDDM TAAPMDVRQW VDTDDTSSEN TVVPPETYVK VAGHLRSFQN KKSLVAFKIM PLEDMNEFTT HILEVINAHM VLSKANSQPS AGRAPISNPG MSEAGNFGGN SFMPANGLTV AQNQVLNLIK ACPRPEGLNF QDLKNQLKHM SVSSIKQAVD FLSNEGHIYS TVDDDHFKST DAE

purity :

> 85 % by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Epigenomics

products description :

Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. The C-terminus of RPA2 can specfically intereact with the DNA repair enzyme uNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, this protein binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA2 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3. Recombinant human RPA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Wang M., et al. (2000) Biochemistry. 39(21):6433-9. Mer G., et al. (2000) Cell. 103(3):449-56.

ncbi gi num :

4506585

ncbi acc num :

NP_002937

ncbi gb acc num :

NM_002946.4

ncbi mol weight :

31.7 kDa (293aa) confirmed by MALDI-TOF

ncbi pathways :

Activation Of ATR In Response To Replication Stress Pathway (105754); Activation Of The Pre-replicative Complex Pathway (105777); Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway (105873); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765); Cellular Response To Heat Stress Pathway (1127553); Cellular Responses To Stress Pathway (645258); Chromosome Maintenance Pathway (161048); DNA Repair Pathway (105837)

uniprot summary :

RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA replication. Chromosomal Location of Human Ortholog: 1p35. Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; DNA replication factor A complex; nucleus. Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; damaged DNA binding; protein N-terminus binding; protein phosphatase binding; single-stranded DNA binding. Biological Process: G1 DNA damage checkpoint; mismatch repair; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

size1 :

0.05 mg

price1 :

375 USD

size2 :

0.25 mg

price2 :

925

more info or order :

MyBioSource product webpage