WHSC2, 1-539aa, Human, His tag, E Coli | MyBioSource MBS203792 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

WHSC2, 1-539aa, Human, His tag, E Coli

catalog :

MBS203792

quantity :

0.05 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203792

products type :

Recombinant Protein

products full name :

WHSC2, 1-539aa, Human, His tag, E Coli

products short name :

WHSC2

products name syn :

Wolf-Hirschhorn syndrome candidate 2; NELF-A; NELFA; P/OKcl.15; Negative elongation factor A

other names :

negative elongation factor A; Negative elongation factor A; negative elongation factor A; wolf-Hirschhorn syndrome candidate 2 protein; negative elongation factor complex member A; Wolf-Hirschhorn syndrome candidate 2 protein

products gene name :

WHSC2

other gene names :

NELFA; NELFA; WHSC2; NELF-A; P/OKcl.15; WHSC2; NELF-A

uniprot entry name :

NELFA_HUMAN

sequence length :

539

sequence :

MGSSHHHHHH SSGLVPRGSH MPGQRRALSP KMASMRESDT GLWLHNKLGA TDELWAPPSI ASLLTAAVID NIRLCFHGLS SAVKLKLLLG TLHLPRRTVD EMKGALMEII QLASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP VKHFQLKRKP KSATLRAELL QKSTETAQQL KRSAGVPFHA KGRGLLRKMD TTTPLKGIPK QAPFRSPTAP SVFSPTGNRT PIPPSRTLLR KERGVKLLDI SELDMVGAGR EAKRRRKTLD AEVVEKPAKE ETVVENATPD YAAGLVSTQK LGSLNNEPAL PSTSYLPSTP SVVPASSYIP SSETPPAPSS REASRPPEEP SAPSPTLPAQ FKQRAPMYNS GLSPATPTPA APTSPLTPTT PPAVAPTTQT PPVAMVAPQT QAPAQQQPKK NLSLTREQMF AAQEMFKTAN KVTRPEKALI LGFMAGSREN PCQEQGDVIQ IKLSEHTEDL PKADGQGSTT MLVDTVFEMN YATGQWTRFK KYKPMTNVS

purity :

> 85% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 20% glycerol

concentration :

0.25 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Transcription & Translation

products description :

WHSC2, also known as NELF-A, is a protein factor required for DRB-sensitive transcription. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Wolf-Hirschhorn syndrome is a multiple malformation syndrome characterized by mental and developmental defects resulting from a hemizygous deletion of the distal short arm of chromosome 4 (4p16.3). Recombinant human WHSC2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Care A., et al. (2007) Nat Med. 13(5):613-8. Mariotti M., et al. (2000) FEBS Lett. 487(2):166-70.

ncbi gi num :

194733742

ncbi acc num :

NP_005654

ncbi gb acc num :

NM_005663.4

ncbi mol weight :

60.6 kDa (559aa)

ncbi pathways :

Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway (106005); Disease Pathway (530764); Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway (161052); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); Formation Of RNA Pol II Elongation Complex Pathway (105950); Formation Of The Early Elongation Complex Pathway (105949); Formation Of The HIV-1 Early Elongation Complex Pathway (106002); Gene Expression Pathway (105937); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984)

ncbi summary :

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]

uniprot summary :

WHSC2: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. Belongs to the NELF-A family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 4p16.3. Cellular Component: nucleoplasm; cytoplasm. Molecular Function: protein binding; chromatin binding. Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; multicellular organismal development; RNA elongation from RNA polymerase II promoter; gene expression

size1 :

0.05 mg

price1 :

375 USD

size2 :

0.25 mg

price2 :

925

more info or order :

MyBioSource product webpage