catalog number :
MBS203791
products type :
Recombinant Protein
products full name :
Ecotin, 21-162aa, E Coli, His tag, E Coli
products short name :
Ecotin
products name syn :
ECK2201; eti; JW2197
other names :
ecotin, a serine protease inhibitor; Ecotin; ecotin, a serine protease inhibitor
other gene names :
eco; eco; ECK2201; eti; JW2197; eti
uniprot entry name :
ECOT_ECOLI
sequence :
MGSSHHHHHH SSGLVPRGSH MAESVQPLEK IAPYPQAEKG MKRQVIQLTP QEDESTLKVE LLIGQTLEVD CNLHRLGGKL ENKTLEGWGY DYYVFDKVSS PVSTMMACPD GKKEKKFVTA YLGDAGMLRY NSKLPIVVYT PDNVDVKYRV WKAEEKIDNA VVR
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 1mM DTT, 10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Proteases & Inhibitors
products description :
Ecotin (E. coli serine protease inhibitor) inhibits pancreatic serine proteases. This protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity. Recombinant E. coli Ecotin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Lilla JN., et al. (2009) J Biol Chem. 284(20):13792-803. Lund LR., et al. (2006) EMBO J. 25(12):2686-97.
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
18.3 kDa (163aa), confirmed by MALDI-TOF
ncbi summary :
Ecotin inhibits trypsin and a number of additional heterologous proteases . [More information is available at EcoCyc: EG10255].