uCK2, 1-261aa, Human, His tag, E Coli | MyBioSource MBS203786 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

uCK2, 1-261aa, Human, His tag, E Coli

catalog :

MBS203786

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203786

products type :

Recombinant Protein

products full name :

uCK2, 1-261aa, Human, His tag, E Coli

products short name :

uCK2

products name syn :

uridine-cytidine kinase 2; TSA903; uK; uMPK

other names :

uridine-cytidine kinase 2; Uridine-cytidine kinase 2; uridine-cytidine kinase 2; cytidine monophosphokinase 2; testis-specific protein TSA903; uridine kinase; uridine monophosphate kinase; uridine monophosphokinase 2; uridine-cytidine kinase 2; Cytidine monophosphokinase 2; Testis-specific protein TSA903; Uridine monophosphokinase 2

products gene name :

uCK2

other gene names :

UCK2; UCK2; UK; UMPK; TSA903; UMPK; UCK 2

uniprot entry name :

UCK2_HUMAN

sequence length :

261

sequence :

MAGDSEQTLQ NHQQPNGGEP FLIGVSGGTA SGKSSVCAKI VQLLGQNEVD YRQKQVVILS QDSFYRVLTS EQKAKALKGQ FNFDHPDAFD NELILKTLKE ITEGKTVQIP VYDFVSHSRK EETVTVYPAD VVLFEGILAF YSQEVRDLFQ MKLFVDTDAD TRLSRRVLRD ISERGRDLEQ ILSQYITFVK PAFEEFCLPT KKYADVIIPR GADNLVAINL IVQHIQDILN GGPSKRQTNG CLNGYTPSRK RQASESSSRP HLEHHHHHH

purity :

> 90% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl, 0.1mM PMSF, 1mM EDTA

concentration :

1mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Enzymes & Proteases

products description :

uCK2 also known as uridine-cytidine kinase 2 is belongs to the uridinekinase family. uCK2 catalyzes the phosphorylation of uridine monophosphate to uridine diphosphate and cytidine monophosphate. It plays a role in the production of pyrimidine nucleoside triphosphates required for RNA and DNA synthesis. In addition, an allele of this gene may play a role in mediating nonhumoral immunity to Hemophilus influenzae type B. Recombinant human uCK2 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Smith AJ., et al. (2009) Org Biomol Chem. 7(13):2716-24. Murata D., et al (2004) Drug Metab Dispos. 32(10):1178-82

ncbi gi num :

18699734

ncbi acc num :

NP_036606

ncbi gb acc num :

NM_012474.4

ncbi mol weight :

30.3kDa (269aa), confirmed by MALDI-TOF

ncbi pathways :

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Fluoropyrimidine Activity Pathway (198778); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309); Pyrimidine Salvage Reactions Pathway (106284)

ncbi summary :

This gene encodes a pyrimidine ribonucleoside kinase. The encoded protein (EC 2.7.1.48) catalyzes phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively.[provided by RefSeq, Oct 2010]

uniprot summary :

UCK2: Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4- thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)- benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5- methylcytidine, and N(4)-anisoylcytidine. Belongs to the uridine kinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.1.48; Xenobiotic Metabolism - drug metabolism - other enzymes; Kinase, other; Nucleotide Metabolism - pyrimidine. Chromosomal Location of Human Ortholog: 1q23. Cellular Component: intracellular membrane-bound organelle; cytosol. Molecular Function: uridine kinase activity; nucleoside kinase activity; ATP binding. Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; CMP salvage; pyrimidine nucleoside salvage; feeding behavior; response to axon injury; phosphorylation

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage